Figure 2: Studying Autophagy with Fluorescence Resonance Energy Transfer (image) Institute for Basic Science Share Print E-Mail Caption Cy3 emits fluorescence with a peak wavelength at 563 nanometers (black line), while Cy5 at 670 nanometers (red line). In the experiment, Cy3 is attached to CB and Cy5 to AdA. During autophagy, the two dyes come closer and Cy3 transfers some energy to its nearby partner dye Cy5, following the FRET phenomenon. Credit IBS Usage Restrictions None Share Print E-Mail Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.