Immunoblot analysis using anti-CKAP4 antibody with whole-cell lysates from A549, RERF-LC-AI, N231, and LCN1, and lung tissue samples. A) CKAP4 protein is detected at approximately 63 kDa in all lung cancer cells. B) CKAP4 protein is also detected at approximately 63 kDa in culture supernatants of A549 and RERF-LC-AI cells. C) The CKAP4 protein is expressed only in lung cancer tissues, and not in their normal counterparts. T: tumor tissue, N: normal lung tissue. CKAP4 was observed at various intensities in the cytoplasm of lung cancer cells (D: A549, E: RERF LC-AI, F: N231, G: LCN1) and tissues (H: AC, I: SCC), but not in normal lung epithelium (J: alveolar epithelium, K: bronchial epithelium).