In vivo effects of treatment with L-Grb2 in combination with anti-angiogenic therapy in an ovarian tumor model. (A-C) Mean tumor weights in (A), numbers of metastatic nodules in (B), and body weights of (C) mice intraperitoneally inoculated with SKOV3ip1 cells that received control treatment, B-20 only (6.25 mg/kg) twice weekly, L-Grb2 only (15 mg/kg) twice weekly, or a combination of B-20 and L-Grb2 beginning 10 days after inoculation (n = 9 mice per group). (D) Tumors collected from the SKOV3ip1 and OVCAR5 models at the conclusion of in vivo therapeutic experiments were subjected to immunohistochemical staining for CD31 to evaluate the effects on tumor vessel number of treatment with L-Grb2, B-20, or both compared with the control treatment. Representative staining images taken at 20× magnification are shown. The mean CD31+ cell numbers per group are shown in the adjoining graphs. Five tumors per group were stained, and five representative images per sample were quantified for analysis. Error bars, SEM. All statistical tests were two-sided. Asterisk indicates statistical significance of ***p < 0.001, **p < 0.01, *p < 0.05. NS indicates non-significant.