UC Davis biomedical engineer ProfESSOR Alexander Revzin has developed a "lab on a chip" device for HIV testing. Revzin's microfluidic device uses antibodies to "capture" white blood cells called T cells that are affected by HIV. In addition to physically binding these cells the test detects the types and levels of inflammatory proteins (cytokines) released by the cells.
Revzin's team collaborated with UCLA electrical engineer Prof. Aydogan Ozcan to integrate an antibody microarray with a lensfree holographic imaging device that takes only seconds to count the number of captured cells and amount of secreted cytokine molecules. The test returns results six to twelve times faster than traditional approaches and tests six parameters simultaneously, based on a small blood sample. The Revzin team published the results of their experiments in the May 2010 issue of Analytical Chemistry.
With further refinements, the test will have wide potential use for multi-parametric blood analysis performed at the point of care in the developing world and resource-poor areas. Its affordability will also make it an attractive option in wealthier areas. Revzin has filed for a patent and is looking for ways to bring his test into clinical use.