(a) This is an overview of Digenome-sequencing. Forward and reverse sequence reads are shown in pink and blue, respectively. Red triangles and vertical lines indicate cleavage positions. WT, wild type.
(b) These are representative IGV images obtained using the HBB-specific RGEN at the on-target site. An indel is indicated by the arrow.
(c) These 5?-end plots showing the absolute and relative number of sequence reads with the same 5? end across nucleotide positions.