Researchers have developed a test that can rapidly and reliably diagnose sepsis, a potentially life-threatening complication of bacterial infections.
Rapid diagnosis of sepsis in hospitalized patients is crucial because in severe cases, there is an average 7.6% decrease in survival rate per hour from the onset of low blood pressure without effective antimicrobial treatment. Early identification of a pathogen increases the chance of targeting the correct agent and may avoid misuse of antibiotics.
In a Journal of Clinical Laboratory Analysis study, investigators describe what is called a TaqMan-Based Multiplex real-time PCR detection system, which allowed for rapid detection of 10 of the most frequent bacterial pathogens from blood samples.
"Interestingly, pathogens in some blood culture-negative cases of sepsis patients were still detected in this study. We speculate that the residual DNA fragments of the bacteria might be detected by this system even if they were destroyed by antibacterial drugs or the immune system," said Dr. Bing Zhang, senior author of the study.
Journal of Clinical Laboratory Analysis