In the study, published in the March 1, 2002 issue of the Journal of Neuroscience, the investigators fed one group of mice with Alzheimer's-like plaques in their brains a diet that included normal amounts of folate, while a second group was fed a diet deficient in this vitamin. . These mice are transgenic, meaning they were bred with mutant genes that cause AD in people. They develop AD-like plaques in their brains that kill neurons.
The NIA team counted neurons in the hippocampus, a brain region critical for learning and memory that is destroyed as plaques accumulate during Alzheimer's disease. The investigators found a decreased number of neurons in the mice fed the folic acid deficient diet. The scientists also discovered that mice with low amounts of dietary folic acid had elevated levels of homocysteine, an amino acid, in the blood and brain. They suspect that increased levels of homocysteine in the brain caused damage to the DNA of nerve cells in the hippocampus. In transgenic mice fed an adequate amount of folate, nerve cells in this brain region were able to repair damage to their DNA. But in the transgenic mice fed a folate-deficient diet, nerve cells were unable to repair this DNA damage.
"These new findings establish a possible cause-effect relationship between elevated homocysteine levels and degeneration of nerve cells involved in learning and memory in a mouse model of Alzheimer's disease," said Mark Mattson, Ph.D., chief of the NIA's Laboratory of Neurosciences and the study's principal investigator.
People who have Alzheimer's disease often have low levels of folic acid in their blood, but it is not clear whether this is a result of the disease or if they are simply malnourished due to their illness. But based on emerging research, Dr. Mattson speculates consuming adequate amounts of folic acid--either in the diet or by supplementation--could be beneficial to the aging brain and help protect it against Alzheimer's and other neurodegenerative diseases. Green leafy vegetables, citrus fruits and juices, whole wheat bread and dry beans are good sources of the vitamin. Since 1998, the Food and Drug Administration has required the addition of folic acid to enriched breads, cereals, flours, corn meals, pastas, rice, and other grain products. However, because it can take a long time for the symptoms of Alzheimer's disease to surface, researchers speculate it will be many years before folate supplementation in food could affect the incidence of dementia in the United States. A human clinical trial is being planned.
In AD, plaques develop first in areas of the brain used for memory and other cognitive functions. They consist of largely insoluble deposits of a protein called beta-amyloid. Although researchers still do not know whether amyloid plaques themselves cause AD or whether they are by-products of the AD process, there is evidence that amyloid deposition may be a central process in the disease. But unlike human brain cells, the brain cells in laboratory mice are not killed by the progressive accumulation of beta amyloid. This finding led Dr. Mattson and his research team to suspect folic acid or some other component of the mouse diet might help these nerve cells resist beta amyloid damage. In earlier work, Dr. Mattson found evidence suggesting folic acid deficiency can increase the brain's susceptibility to Parkinson's disease.
The NIA leads the Federal effort to support and conduct basic, clinical, and social and behavioral studies on aging and AD. It supports the Alzheimer's Disease Education and Referral (ADEAR) Center, which provides information on AD research, including clinical trials, to the public, health professionals, and the media. ADEAR can be contacted toll free at 1-800-438-4380 weekdays or by visiting the website www.alzheimers.org. Press releases, fact sheets, and other materials about aging and aging research can be viewed at the NIA's general information website, www.nia.nih.gov.
* I. Kruman, T.S. Kumaravel, A. Lohani, W. Pedersen, R.G. Cutler, Y. Kruman, N. Haughey, J. Lee, M. Evans, and M.P. Mattson, "Folic Acid Deficiency and Homocysteine Impair DNA Repair in Hippocampal Neurons and Sensitize Them To Amyloid Toxicity in Experimental Models of Alzheimer's Disease," Journal of Neuroscience, 22:5, pp. 1752-1762.
¨ S. Sesdradri, A. Beiser, J. Selhub, et al., "Plasma Homocysteine As A Risk Factor For Dementia and Alzheimer's Disease," N Eng J Med, 346:7, pp. 476-483.