The mothers normally have a rheumatic disease, such as Sjögren's syndrome or SLE. In virtually all cases of congenital heart block, the mother produces antibodies against the Ro and La autoantigens, but in only 2-5 per cent of pregnancies amongst Ro and La positive women does this heart condition arise.
This study has identified the specific antibody that causes the damage, and explained its mechanism of action. The antibody targets a specific part of the Ro protein known as p200. The study shows that the level of p200 antibodies in the mother correlates with the degree of damage to the heart. The results have been further confirmed in studies on rats and in cell cultures. Cultivated heart cells, cardiomyocytes, were seriously affected in their calcium balance when exposed to specific p200 antibodies. The cells were rendered unable to accumulate calcium, which ultimately led to cell death, apoptosis.
Knowledge of how this congenital heart condition arises will go a long way towards helping scientists develop better screening, risk assessment and treatment techniques. This is particularly important as early intervention has proved to restrict the extent of the damage, thus preventing pacemaker dependence or foetal death.
"We believe that the antibody we've identified can be used as a marker for identifying the mothers who are at a high risk of developing this complication during pregnancy," says associate professor Marie Wahren-Herlenius. "We're now working together with Karolinska University Hospital on designing a suitable method for this."
Publication:
Ro/SSA autoantibodies directed bind cardiomyocytes, disturb calcium homeostasis and mediate congenital heart block
Salomonsson S, Sonesson S-E, Ottosson L, Muhallab S, Olsson T, Sunnerhagen M, Kuchroo VK, Thorén P, Herlenius E, Wahren-Herlenius M.
Journal of Experimental Medicine (2005) 201: 11-17.
For more information, please contact:
Associate professor Marie Wahren-Herlenius, Karolinska Institutet, Sweden, phone +46 8 5177 6746 or e-mail marie.wahren@cmm.ki.se
Journal
Journal of Experimental Medicine