The research appears as the "Paper of the Week" in the March 25 issue of the Journal of Biological Chemistry, an American Society for Biochemistry and Molecular Biology journal.
The innate immune system uses the CD14 receptor protein to recognize several microbial and cellular products including lipopolysaccharide (LPS), a glycolipid found on the outer membrane of certain bacteria. Once CD14 binds to LPS or another ligand, it presents the molecule to other proteins which initiate a strong pro-inflammatory response that stimulates host defenses.
"Macrophages and monocytes can recognize distinct structural patterns in various molecules from pathogenic microorganisms," explains Dr. Jie-Oh Lee of the Korea Advanced Institute of Science and Technology. "LPS is the most famous and probably the most important inducer of the innate immune response."
Dr. Lee and his colleagues solved the three-dimensional crystal structure of CD14, providing crucial insights into how the receptor binds to its ligands. "Our structure shows that CD14 has a large hydrophobic pocket near its amino terminus," says Dr. Lee. "We propose that this pocket is the main binding site for LPS because previous biochemical studies demonstrate that amino acid residues comprising the pocket are critical for LPS binding. Most, if not all, of the CD14 ligands compete with LPS for CD14 binding. Therefore, they probably share the same binding pocket with LPS."
Ligands other than LPS can be accommodated in the pocket due to its large size, the flexibility of its rim, and the multiple grooves available for ligand binding. The researchers also discovered that mutations that interfere with LPS signaling cluster in a separate area near the pocket suggesting that the areas around the pocket are important in LPS transfer.
Not only do these findings shed light on how cells recognize pathogens, they also may also lead to the development of drugs to help treat septic shock, an often fatal systemic bacterial infection that is triggered by LPS.
"Pharmaceutical companies have tried to develop anti-septic shock agents for a long time without clear success," explains Dr. Lee. "Since LPS is an important inducer of septic shock, blocking LPS receptors such as CD14 are among the most important targets. Our structure shows the shape of the LPS binding pocket of CD14. Now, drug developers will have better chance to design a molecule that will complement the shape of the pocket."
The Journal of Biological Chemistry's Papers of the Week is an online feature which highlights the top one percent of papers received by the journal. Brief summaries of the papers and explanations of why they were selected for this honor can be accessed directly from the home page of the Journal of Biological Chemistry online at www.jbc.org.
The American Society for Biochemistry and Molecular Biology (ASBMB) is a nonprofit scientific and educational organization with over 11,000 members in the United States and internationally. Most members teach and conduct research at colleges and universities. Others conduct research in various government laboratories, nonprofit research institutions, and industry.
Founded in 1906, the Society is based in Bethesda, Maryland, on the campus of the Federation of American Societies for Experimental Biology. The Society's primary purpose is to advance the sciences of biochemistry and molecular biology through its publications, the Journal of Biological Chemistry, The Journal of Lipid Research, Molecular and Cellular Proteomics, and Biochemistry and Molecular Biology Education, and the holding of scientific meetings.
For more information about ASBMB, see the Society's website at www.asbmb.org.