COLD SPRING HARBOR, N.Y. (Tues., Sept. 4, 2007) - Skin cancer is the most prevalent form of human cancer in the United States, according to the Centers for Disease Control. But in order to more fully understand skin cancer in humans, scientists must use model organisms, such as mice, to study the disease in the laboratory.
This month's release of Cold Spring Harbor Protocols (www.cshprotocols.org) includes free access to a protocol for generating mice with squamous cell carcinoma (SCC), one of the most common types of skin cancer. The procedure involves injecting mice with a drug called DMBA, which mutates (and thereby activates) a tumor-promoting gene. A second drug, called TPA, then encourages the proliferation of cells that carry the mutated gene. The resulting mass of cells is a tumor.
The protocol, freely available at http://www.
The protocol is from Dr. Michael Girardi's group at the Yale University School of Medicine (http://info.
Also freely available from Cold Spring Harbor Protocols this month is an article that describes an efficient method for testing individuals for specific DNA variations called SNPs (single-nucleotide polymorphisms) (http://www.
About Cold Spring Harbor Protocols: Cold Spring Harbor Protocols (www.cshprotocols.org) is an online resource of methods used in a wide range of biology laboratories. It is structured to be highly interactive, with each protocol cross-linked to related methods, descriptive information panels, and illustrative material to maximize the total information available to investigators. Each protocol is clearly presented and designed for easy use at the bench--complete with reagents, equipment, and recipe lists. Life science researchers can access the entire collection via institutional site licenses, and can add their suggestions and comments to further refine the techniques.
About Cold Spring Harbor Laboratory Press: Cold Spring Harbor Laboratory Press is an internationally renowned publisher of books, journals, and electronic media, located on Long Island, New York. Since 1933, it has furthered the advance and spread of scientific knowledge in all areas of genetics and molecular biology, including cancer biology, plant science, bioinformatics, and neurobiology. It is a division of Cold Spring Harbor Laboratory, an innovator in life science research and the education of scientists, students, and the public. For more information, visit www.cshlpress.com.