WORCESTER, Mass. - Researchers at the University of Massachusetts Medical School have described a previously unknown role for the cilia protein IFT88 in mitosis, the process by which a dividing cell separates its chromosomes containing the cell's DNA into two identical sets of new daughter cells. Published in advance online by Nature Cell Biology, this newly discovered function for IFT88 suggests a possible alternative or contributory cause for cilia-related diseases such as primary ciliary dyskinesia, and polycystic kidney disease.
Famous for its ability to build cilia, a slender protrusion responsible for motility and sensory input, IFT88 is part of a family of transport proteins and cellular machinery that is responsible for moving materials from the cell body to the cilia. These cellular materials are necessary for the proper formation and maintenance of cilia and in the absence of IFT88 cilia are either unable to form or are defective. Over the last several years, scientists have linked cilia dysfunction to a number of diseases now known as ciliopathies. In particular, the loss of IFT88, one of the best-studied cilia proteins, has been associated with polycystic kidney disease (PKD). PKD is characterized by the presence of multiple cysts in the kidneys and is believed to be caused by cilia dysfunction in kidney cells.
Stephen J. Doxsey, PhD, professor of molecular medicine and biochemistry & molecular pharmacology and cell biology and lead author of the study, shows that IFT88 also plays an important role in mitosis. Doxsey and colleagues at UMass Medical School observed that the IFT88 protein is present at the poles of the mitotic spindle, structures which form during mitosis and are required to guide chromosome sets as the cell divides in two and to orient the plane of cell division. He hypothesized that the cilia protein played a transport function during mitosis that was similar to that in cilia.
"We knew that IFT88 and several other proteins in this family were present at spindle poles during mitosis but we didn't know what function, if any, they served," said Doxsey."
Benedicte Delaval, PhD, a postdoctoral fellow in Doxsey's lab and first author of the Nature Cell Biology paper added, "What we found is that IFT88 plays a part in the transport of materials required for building the spindle poles during cell division. The loss of IFT88 protein during mitosis caused a delay in mitotic division and misalignment of the direction and plane of cell division.
This finding raises the possibility that mitotic dysfunction may play a role in previously described cilia-related disorders caused by the same family of IFT proteins. "It appears that the cellular machinery used in cilia formation is very similar to the machinery used in mitosis," said Doxsey. "This new and distinct function for IFT88 suggests that disruption of both cilia and mitosis could co-contribute to ciliopathies."
Since both cilia and spindles arise from a structure in the cell called the centrosome, Doxsey hypothesizes that there may be some underlying defect to the centrosome responsible for both processes going awry. "It's possible that cilia defects alone might actually explain only part of the disease - especially in the kidney," said Doxsey. "At this point, we don't know. What we do know is that IFT88's role in mitosis has yet to be fully explored. It appears, though, to play a similar role in two different functions. We need to take a more global look at the cell to determine what precisely is going on in this family of disorders."
About the University of Massachusetts Medical School
The University of Massachusetts Medical School, one of the fastest growing academic health centers in the country, has built a reputation as a world-class research institution, consistently producing noteworthy advances in clinical and basic research. The Medical School attracts more than $255 million in research funding annually, 80 percent of which comes from federal funding sources. The work of UMMS researcher Craig Mello, PhD, an investigator of the prestigious Howard Hughes Medical Institute (HHMI), and his colleague Andrew Fire, PhD, then of the Carnegie Institution of Washington, toward the discovery of RNA interference was awarded the 2006 Nobel Prize in Medicine and has spawned a new and promising field of research, the global impact of which may prove astounding. UMMS is the academic partner of UMass Memorial Health Care, the largest health care provider in Central Massachusetts. For more information, visit www.umassmed.edu.