Opening up a new door in synthetic biology, a team of researchers has developed a microfluidic device that produces a continuous supply of tiny lipid spheres that are similar in many ways to a cell's outer membrane. "Cells are essentially small, complex bioreactors enclosed by phospholipid membranes," said Abraham Lee from the University of California, Irvine. "Effectively producing vesicles with lipid membranes that mimic those of natural cells is a valuable tool for fundamental biology research, and it's also an important first step in the hoped-for production of an artificial cell." The researchers have taken an important step in advancing this field by developing a single system that quickly and efficiently performs all the necessary steps to create stable lipid vesicles. Current multistep production methods create vesicles that have inconsistent sizes and layers and short usable lifespans, and they are often contaminated with solvents used in their production.
A paper accepted for publication in the AIP's journal.
Biomicrofluidics reports that the new microfluidic design overcomes these previous hurdles by generating and manipulating precisely sized droplets of water in an oil environment. This produces an oil-and-water membrane that serves as a scaffold around which lipids molecules assemble. As the membrane dissolves over time, the accumulated lipids form a stable, uniform vesicle that shares many of a natural cell membrane's chemical and physical attributes.
Article: "Stable, Biocompatible Lipid Vesicle Generation by Solvent Extraction-based Droplet Microfluidics" is accepted for publication in the journal Biomicrofluidics.
Authors: Shai-Yen The (1), Ruba Khnouf (2), Hugh Fan (2, 3), Abraham Lee (1, 4).
(1) Department of Biomedical Engineering, University of California at Irvine
(2) Department of Biomedical Engineering, University of Florida
(3) Department of Mechanical and Aerospace Engineering, University of Florida
(4) Department of Mechanical and Aerospace Engineering, University of California at Irvine