image: The toxins normally bind very strongly to the antitoxins and are thus not only inactive, but also prevent the production of more toxin from the information encoded in the bacterial DNA. During the dormant state, however, the antitoxins are degraded, and the toxins released (step 1). The free toxins now bind to unoccupied antitoxins on DNA within the area encoding the toxin-antitoxin couple (step 2). Binding increasing amounts of toxin eventually leads to the release of the molecules from the gene (steps 3 and 4) and finally to new toxin production. view more
Credit: Ditlev E. Brodersen
Many pathogenic bacteria are able to go into a dormant state by producing persister cells that are not susceptible to conventional antibiotics. This causes serious problems in the treatment of life-threatening diseases such as tuberculosis, where the presence of persister cells often leads to a resurgence of infection following medical treatment.
At the molecular level, the formation of persister cells is due to the presence of toxins that are produced by the bacteria themselves, and which enable them to enter the dormant state. During this hibernation period, the bacteria constantly regulate the amount of toxin at exactly the same level and thus maintain the dormant state.
In an article recently published in the American scientific journal Structure, the researchers at the Department of Molecular Biology and Genetics, Aarhus University, present new results that reveal the molecular details of the regulatory mechanism of toxins.
By isolating and crystallising the toxin molecules and their molecular companions – the antitoxins – and by subsequently exposing the crystals to strong X-rays, the research team (consisting of the two PhD students Andreas Bøggild and Nicholas Sofos and Associate Professor Ditlev E. Brodersen) gained unique insight into how bacteria control the amount of toxin in the cell (illustrated in the info box).
The new findings can eventually lead to the development of entirely new forms of treatment of bacterial infections that work initially by blocking toxin function and production, and subsequently by using traditional antibiotics to fight the pathogenic bacteria.
Journal
Structure