Tampa, Fla. (Sep. 25, 2012) – In a study to determine the best cryopreservation (freezing) solution to maintain induced pluripotent stem (iPS) cells, a team of researchers from Japan compared 12 kinds of commercially prepared and readily available cryopreservation solutions and found that "Cell Banker 3" out-performed the other 11 solutions by allowing iPS cells to be preserved for a year at degrees C in an undifferentiated state.
The study is published in a recent special issue of Cell Medicine [3(1)], now freely available on-line at: http://www.ingentaconnect.com/content/cog/cm.
"iPS cells are a promising alternative to embryonic stem cells and can be used in place of bone marrow cells, stromal cells and adipose tissue-derived stem cells," said study co-author Hirofumi Noguchi, MD, PhD, Department of Gastroenterological Surgery, Transplant and Surgical Oncology at the Okayama University Graduate School of Medicine. "However, the viability of human iPS cells, like embryonic stem cells, decreases significantly during cryopreservation. A wide variety of cryopreservation solutions have been used, however many are toxic or ineffective for use in extended cryopreservation."
The researchers concluded that Cell Banker 3 showed the highest cell viability and proliferation of all the solutions examined and can be widely used as it does not require any special skills for use.
This research was among those studies presented at the 37th Annual Meeting of the Japan Society for Organ Preservation and Medical Biology (JSOPMB). Sixteen studies were published in this special issue of CELL MEDICINE. The theme of the issue is "Organ/Cell Transplantation and Regenerative Medicine."
Citation: Miyamoto, Y.; Noguchi, H.; Yukawa, H.; Oishi, K.; Matsushita, K.; Iwata, H.; Hayashi, S. Cryopreservation of Induced Pluripotent Stem Cells. Cell Med. 3(1):89-95; 2012.
Contact: Dr. Hirofumi Noguchi, Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata, Okayama 700-8558 Japan
Tel + 81-86-235-7257; Fax + 81-86-221-8775
Noguchih2006@yahoo.co.jp / noguch-h@cc.okayama-u.ac.jp
The editorial offices for CELL MEDICINE are at the Center of Excellence for Aging and Brain Repair, College of Medicine, the University of South Florida. Contact, David Eve, PhD. at cellmedicinect@gmail.com
News Release by Florida Science Communications
Journal
Cell Medicine