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A single-domain antibody that specifically recognizes amyloid-beta 42 oligomers

Neural Regeneration Research


IMAGE: The anti-beta-amyloid monoclonal antibody 6E10 could recognize all types of Aβ42, but clone 10A1 and 11F5 only bound to tetramers and octamers. The helper phage M13K07 was used as a... view more

Credit: Neural Regeneration Research

Earlier amyloid-beta assemblies may be one of the most important causes of Alzheimer's disease. Passive immunization of anti-amyloid-beta antibodies can reduce amyloid-beta burden and improved memory performance in various Alzheimer's disease animal models or patients. Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, a recent study published in the Neural Regeneration Research (Vol. 8, No. 33, 2013) successfully constructed a naïve human Fab library and obtained a human Fab antibody and a single-domain antibody that specifically recognized amyloid-beta (Aβ)42 oligomers.


Article: " Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers," by Zuanning Yuan1, 2, Minge Du1, 2, Yiwen Chen3, Fei Dou1, 2 (1 Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Beijing 100875, China; 2 Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Southeast University, Nanjing 210009, Jiangsu Province, China; 3 Geriatric Internal Department of Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China)

Yuan ZN, Du MG, Chen YW, Dou F. Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers. Neural Regen Res. 2013;8(33):3107-3115.

Contact: Meng Zhao
Neural Regeneration Research

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