Earlier amyloid-beta assemblies may be one of the most important causes of Alzheimer's disease. Passive immunization of anti-amyloid-beta antibodies can reduce amyloid-beta burden and improved memory performance in various Alzheimer's disease animal models or patients. Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, a recent study published in the Neural Regeneration Research (Vol. 8, No. 33, 2013) successfully constructed a naïve human Fab library and obtained a human Fab antibody and a single-domain antibody that specifically recognized amyloid-beta (Aβ)42 oligomers.
Article: " Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers," by Zuanning Yuan1, 2, Minge Du1, 2, Yiwen Chen3, Fei Dou1, 2 (1 Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Beijing 100875, China; 2 Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Southeast University, Nanjing 210009, Jiangsu Province, China; 3 Geriatric Internal Department of Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China)
Yuan ZN, Du MG, Chen YW, Dou F. Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers. Neural Regen Res. 2013;8(33):3107-3115.