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JCI online ahead of print table of contents for March 25, 2014

JCI Journals

Epigenetic alterations disrupt intestinal T cell homeostasis

A precise balance between mature T cell subsets is important for intestinal homeostasis. Disruption of T cell populations underlies autoimmune colitis, including inflammatory bowel disease (IBD). Specific transcriptional programs are activated to determine the differentiation fate of naïve T cells; however, the role of epigenetic regulation in T cell maturation in the intestine is unclear. In this issue of the Journal of Clinical Investigation, Colby Zaph and colleagues from the University of British Columbia used a murine T cell transfer model, to demonstrate that T cell-specific expression of the histone lysine methyltransferase G9A is required for pathogenic T cell responses and colitis progression. G9A expression in naïve T cells prevented development of Th17 and Treg subsets and was associated with increased histone methylation. Pharmacologic inhibition of G9A restored the ability of T cells to differentiate into Treg and Th17 subsets, suggesting that targeting G9A may relieve intestinal inflammatory disease.

TITLE: Methyltransferase G9A regulates T cell differentiation during murine intestinal inflammation

The University of British Columbia, Vancouver, BC, CAN
Phone: 604-822-7231; Fax: 604-822-7815; E-mail:

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Researchers develop a conditionally immortalized human β cell line

Pancreatic β cells maintain glucose homeostasis through storage and release of insulin. Diabetic patients exhibit drastic reduction and dysfunction in β cell populations, which cannot be regenerated or restored. Most of our current understanding of β cell mass regulation comes from experiments done with murine β cells; however, due to the limited availability of primary human β cells in depth analysis on these cells has been a challence. In this issue of the Journal of Clinical Investigation, Philippe Ravassard and colleagues at Hôpital Pitié Salpêtrière generated a conditionally immortalized human β cell line (EndoC-βH2) that can be greatly expanded before inducing excision of the immortalizing transgenes. Following excision, gene expression profiles of EndoC-βH2 cells were similar to isolated human pancreatic islets. Furthermore, excised EndoC-βH2 cells produced and stored insulin, which was released upon glucose stimulation. As representative human β cells, EndoC-βH2 cell provide a unique tool to further examine human-specific β cell properties and proliferation.

TITLE: Development of a conditionally immortalized human pancreatic β cell line

AUTHOR CONTACT: Philippe Ravassard
Phone: 33 1 57 27 45 75; E-mail:

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TITLE: CaMK4-dependent activation of AKT/mTOR and CREM-α underlies autoimmunity-associated Th17 imbalance

Beth Israel Deaconess Medical Center, Boston, MA, USA
Phone: 617-735 4160 (Betty Chase); E-mail:

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TITLE: PD-1 identifies the patient-specific CD8+ tumor-reactive repertoire infiltrating human tumors

AUTHOR CONTACT: Steven Rosenberg
National Institutes of Health, NIH, National Cancer Institute, Bethesda, MD, USA
Phone: 301-496-4164; E-mail:

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TITLE: Sphingosine-1-phosphate receptor 1 reporter mice reveal receptor activation sites in vivo

NIDDK/NIH, Bethesda, MD, USA
Phone: 301-435-6504; E-mail:

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TITLE: RIP140 increases APC expression and controls intestinal homeostasis and tumorigenesis

AUTHOR CONTACT: Vincent Cavailles
IRCM, Institut de Recherche en cancérologie de Montpellier, Montpellier, FRA
Phone: 33 4 67 61 24 05; E-mail:

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TITLE: Leptin-promoted cilia assembly is critical for normal energy balance

Department of Internal Medicine University of Ulsan College of Medicine, Seoul, KOR
Phone: 82-2-3010-3245; E-mail:

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