Public Release: 

Scientists reveal parchment's hidden stories

The new technique of analyzing DNA found in ancient parchments can shine a focused light on the development of agriculture across the centuries

Trinity College Dublin

Dublin, IRELAND Monday December 8th, 2014 - Millions of documents stored in archives could provide scientists with the key to tracing agricultural development across the centuries, according to new research completed at Trinity College Dublin and the University of York.

Amazingly, thanks to increasingly progressive genetic sequencing techniques, the all-important historical tales these documents tell are no longer confined to their texts; now, vital information also comes from the DNA of the parchment on which they are written.

Researchers used these state-of-the-art scientific techniques to extract ancient DNA and protein from tiny samples of parchment from documents from the late 17th and late 18th centuries. The resulting information enabled them to establish the type of animals from which the parchment was made, which, when compared to genomes of their modern equivalents, provides key information as to how agricultural expansion shaped the genetic diversity of these animals.

This information therefore gives the scientists an unrivalled resource to analyse the development of livestock husbandry across the centuries. The research has just been published in the international, peer-reviewed journal Philosophical Transactions of the Royal Society B.

Professor of Population Genetics at Trinity College Dublin, Daniel Bradley, said: "This pilot project suggests that parchments are an amazing resource for genetic studies that consider agricultural development over the centuries. There must be millions stored away in libraries, archives, solicitors' offices and even in our own attics. After all, parchment was the writing material of choice for thousands of years, going back to the Dead Sea Scrolls."

He added: "Wool was essentially the oil of times gone by, so knowing how human change affected the genetics of sheep through the ages can tell us a huge amount about how agricultural practices evolved."

To conduct their research, geneticists at Trinity extracted DNA from two tiny (2x2cm) samples of parchment provided by the University of York's Borthwick Institute for Archives. Meanwhile, researchers in the Centre for Excellence in Mass Spectrometry at York extracted collagen (protein) from the same parchment samples.

The first sample showed a strong affinity with northern Britain, specifically the region in which black-faced breeds such as Swaledale, Rough Fell and Scottish Blackface are common, whereas the second sample showed a closer affinity with the Midlands and southern Britain where the livestock Improvements of the later 18th century were most active.

If other parchments show similar levels of DNA content, resulting sequencing could provide insights into the breeding history of livestock - particularly sheep - before, during and after the agricultural improvements of the 18th century that led to the emergence of regional breeds of sheep in Britain.

Professor Matthew Collins, of the Department of Archaeology at York, who heads the University's BioArCh research centre, said: "We believe the two specimens derive from an unimproved northern hill-sheep typical in Yorkshire in the 17th century, and from a sheep derived from the 'improved' flocks, such as those bred in the Midlands by Robert Bakewell, which were spreading through England in the 18th century. We want to understand the history of agriculture in these islands over the last 1,000 years and, with this breath-taking resource, we can."

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The research was funded by a grant from the European Research Council. The paper can be viewed here after embargo: http://rstb.royalsocietypublishing.org/content/370/1660.toc

Media Contact:

Daniel Bradley, Professor of Population Genetics, Trinity College Dublin, at dbradley@tcd.ie, or Tel: +353-87-207-9451

Thomas Deane, Press Officer for the Faculty of Engineering, Mathematics, and Science, Trinity College Dublin, at deaneth@tcd.ie, or Tel: +353-1-896-4685

Matthew Collins, Professor of Archaeology, University of York, at matthew.collins@york.ac.uk, or Tel: +44-7955-888101

David Garner, Head of Media Relations, University of York, at david.garner@york.ac.uk or Tel: +44-1904-322153

Additional Notes to Editor

Parchment a Huge Reservoir of DNA Information

Before the mass production of paper, parchment was the major medium for legal documents and until the widespread adoption of typewriters, they were a clerk's preferred medium for many formal legal documents and records.

Parchment has a number of properties that make it compelling material for DNA extraction and analysis. Parchments are made from the skins of domestic animals, particularly livestock, and their manufacture results in robust artefacts, which can survive intact for many centuries.

Secondly, they are abundant and because of their enduring legal value they have generally been carefully managed. In the 20th century, this has included protecting them from both high temperatures and fluctuating humidity.

Thirdly, unlike bone remains, of which only a small percentage have been excavated, parchments are above ground, archived and in the case of legal documents, directly related to specific dates - a level of resolution largely unachievable with any other historic DNA source. Even undated documents can be dated palaeographically to a resolution better and more cheaply than radiocarbon dating.

About Trinity College Dublin

Trinity College Dublin, founded in 1592 is Ireland's oldest university and today has a vibrant community of 17,000 students. It is recognised internationally as Ireland's premier university. Cutting-edge research, technology and innovation places the university at the forefront of higher education in Ireland and globally.

It encompasses all major academic disciplines, and is committed to world-class teaching and research across the range of disciplines in the arts, humanities, engineering, science, social and health sciences.

Trinity is Ireland's leading university across all international rankings, and was ranked 61st globally in 2013 QS World University Rankings

http://www.tcd.ie.

** Note there is video available here: http://youtu.be/f0e-rmri89M **

** Note there are also images and captions available here: http://www.york.ac.uk/news-and-events/features/2014/parchment-hidden-story

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