A collaborative team from The University of Texas Medical Branch at Galveston, Vanderbilt University and The Scripps Research Institute have identified mechanisms involved in antibody response to the deadly Marburg virus by studying the blood of a Marburg survivor. This study now appears online and will be in the Feb. 26 edition of Cell.
Using blood samples from a Marburg survivor, the researchers were able to determine how a person's immune system can fight against the virus.
In the study, researchers investigated the human immune response to Marburg virus, which is a close relative of the Ebola virus. The researchers isolated blood cells of an American who was infected with the Marburg virus several years ago during a visit to a cave in Uganda that is home to Egyptian fruit bats, some of which are now known to carry Marburg. After returning to the U.S., this person developed a very severe, but not fatal, case of Marburg infection. The researchers used the survivor's blood to isolate a large number of B cells that produce antibodies, which are small protein molecules capable of inactivating the virus.
Using a combination of methods, the researchers localized the site on the virus where antibodies were found to bind. This appeared to be the same spot thought to interact with human cells targeted by the virus during the initial phase of infection.
The study shows that the human immune system can effectively fight Marburg virus infections by producing antibodies and shows how these antibodies inactivate the virus. Understanding these mechanisms will help researchers to develop effective antibody-based treatments against both Marburg and Ebola viruses.
"Three years ago, when we started this collaborative work with James Crowe's laboratory at Vanderbilt University, not much was known about the mechanisms of antibody immune response to the filoviruses Marburg and Ebola," said virologist Alex Bukreyev, professor at UTMB and co-senior author. "It was even unclear whether an infected person can develop an effective antibody response to these infections. During these years, the whole area of research moved forward dramatically."
Other authors of this paper include Philipp Ilinykh, Xiaoli Shen, Tania Garron, Thomas Ksiazek and Curtis Klages from the University of Texas Medical Branch; Andrew Flyak, James Slaughter and Gopal Sapparapu from Vanderbilt University and Charles Murin, Marnie Fusco, Takao Hashiguchi, Zachary Bornholdt, Andrew Ward and Erica Ollmann Saphire from The Scripps Research Institute.
This study was supported by the Defense Threat Reduction Agency and the National Institutes of Health.