New Rochelle, NY, May 18, 2015--Researchers seeded type II lung epithelial cells into a decellularized lung matrix to study their function and report the unexpected finding that instead of differentiating into type I lung cells, they instead transitioned to become mesenchymal cells, as would occur in wound healing. The design and results of this study and its implications for the development of protocols and cell culture environments to support the growth of functional lung tissue are presented in an article in Tissue Engineering, Part A, a peer-reviewed journal from Mary Ann Liebert, Inc., publishers. The article is available free on the Tissue Engineering website until June 16, 2015.
Elizabeth Calle and coauthors from Yale University and Yale University School of Medicine, New Haven, CT and University of North Carolina, Chapel Hill, NC, emphasize the effect that factors such as the use of one or more cell populations to seed a tissue matrix, the components of the growth medium, and the use of stimuli such as ventilation to achieve a physiologically appropriate environment can have on the growth and maturation of the lung tissue.
In the article "Fate of Distal Lung Epithelium Cultured in a Decellularized Lung Extracellular Matrix" the authors describe the type II lung epithelial cells isolated from rats and seeded onto decellularized rat lung scaffolds as having migratory, contractile, and matrix-secreting properties after one week, which is atypical of epithelial cells. The cells also had increased expression of markers consistent with mesenchymal cell types. In contrast, the authors report that this type of epithelial-to-mesenchymal transition did not occur when mixed populations of rat cells were seeded on the same scaffold using the same media.
"Scientists reseeding tissue-specific cells onto decellularized extracellular matrices derived from their tissue of origin should be aware that tissue-specific differentiation states should not be assumed to be constant," says Peter C. Johnson, MD, Vice President, Research and Development and Medical Affairs, Vancive Medical Technologies and President and CEO, Scintellix, LLC, Raleigh, NC. "Appropriate testing of cellular markers after reseeding will be essential to characterize the true fate of replaced cells."
About the Journal
Tissue Engineering is an authoritative peer-reviewed journal published monthly online and in print in three parts: Part A, the flagship journal published 24 times per year; Part B: Reviews, published bimonthly, and Part C: Methods, published 12 times per year. Led by Co-Editors-In-Chief Antonios Mikos, PhD, Louis Calder Professor at Rice University, Houston, TX, and Peter C. Johnson, MD, Vice President, Research and Development and Medical Affairs, Vancive Medical Technologies, an Avery Dennison business, and President and CEO, Scintellix, LLC, Raleigh, NC, the Journal brings together scientific and medical experts in the fields of biomedical engineering, material science, molecular and cellular biology, and genetic engineering. Tissue Engineering is the official journal of the Tissue Engineering & Regenerative Medicine International Society (TERMIS). Complete tables of content and sample issues may be viewed online at the Tissue Engineering website.
About the Publisher
Mary Ann Liebert, Inc., publishers is a privately held, fully integrated media company known for establishing authoritative peer-reviewed journals in many promising areas of science and biomedical research, including Stem Cells and Development, Human Gene Therapy, and Advances in Wound Care. Its biotechnology trade magazine, Genetic Engineering & Biotechnology News (GEN), was the first in its field and is today the industry's most widely read publication worldwide. A complete list of the firm's 80 journals, books, and newsmagazines is available on the Mary Ann Liebert, Inc., publishers website.