This news release is available in French.
Chemical substances that are safe for humans when taken in isolation can become harmful when they are combined. Three research teams bringing together researchers from Inserm and CNRS in Montpellier have elucidated in vitro a molecular mechanism that could contribute to the phenomenon known as the "cocktail effect." This study is published in the journal Nature Communications.
Every day we are exposed to many exogenous compounds such as environmental pollutants, drugs or substances in our diet. Some of these molecules, known as endocrine disruptors, are strongly suspected of interacting inappropriately with regulatory proteins in our cells, and inducing numerous physiological or metabolic disorders (cancers, obesity, diabetes, etc.). Moreover, the combination of these molecules in complex mixtures with which we are in routine contact might exacerbate their toxicity.
In an article to be published in Nature Communications, researchers have unveiled a mechanism that might contribute to this effect of mixing, for which no rational explanation has been offered until now. They show that some oestrogens such as ethinyloestradiol (one of the active ingredients of contraceptive pills) and organochlorine pesticides such as trans-nonachlor, although very weakly active on their own, have the ability to bind simultaneously to a receptor located in the cell nucleus, and to activate it synergistically.
Analyses at molecular level indicate that the two compounds bind cooperatively to the receptor, i.e. binding of the first molecule promotes binding of the second. This cooperativity is due to strong interactions at the level of the receptor binding site, so that the binary mixture induces a toxic effect at substantially lower concentrations than the individual molecules.
These results obtained in vitro constitute a proof of concept that opens the way to a wide field of study. There are actually about 150,000 compounds in our environment that could have unexpected effects on human health through combined action, given their recognised or assumed safety as isolated substances. If these studies are confirmed in vivo, important consequences are expected in the areas of endocrine disruption, toxicology, and the assessment of risks associated with the use of chemicals.
Figure: Separately, ethinyloestradiol (EE2) and trans-nonachlor (TNC) bind to the xenobiotic receptor (PXR) only at high concentrations, and are weak activators of this receptor. When they are used together, the two compounds mutually stabilise each other in the binding pocket of the receptor. The "supramolecular ligand" thus created has increased affinity for PXR, so that it can induce a toxic effect at doses at which each compound is inactive individually.
Synergistic activation of human pregnane X receptor by binary cocktails of pharmaceutical and environmental compounds
Vanessa Delfosse1-3,13, Béatrice Dendele3-6,13, Tiphaine Huet1-3,13, Marina Grimaldi3-6, Abdelhay Boulahtouf3-6, Sabine Gerbal-Chaloin3,7, Bertrand Beucher3,8,9, Dominique Roecklin10, Christina Muller10, Roger Rahmani11, Vincent Cavaillès3-6, Martine Daujat-Chavanieu3,7,12, Valérie Vivat10, Jean-Marc Pascussi3,8,9, Patrick Balaguer3-6,14* & William Bourguet1-3,14*
1 Inserm U1054, Montpellier, France;
2 CNRS UMR5048, Centre de Biochimie Structurale, Montpellier, France;
3 Université de Montpellier, Montpellier, France;
4 IRCM, Institut de Recherche en Cancérologie de Montpellier, Montpellier, France;
5 Inserm, U1194, Montpellier, France;
6 ICM, Institut régional du Cancer de Montpellier, Montpellier, France;
7 Inserm U1040, Montpellier, France;
8 Inserm U661, Montpellier, France;
9 CNRS UMR5203, Institut de Génomique Fonctionnelle, Montpellier, France;
10 NovAliX, Illkirch, France;
11 INRA UMR 1331, TOXALIM, Sophia-Antipolis, France;
12 CHU de Montpellier, Institut de Recherche en Biothérapie, Montpellier, France
13 These authors equally contributed to this work
14 These authors jointly supervised this work
Inserm Research Director
Centre for Structural Biochemistry (CBS)
CNRS UMR 5048, Inserm U1054, Montpellier University
+ 33 (0)4 67 41 77 02
Inserm Research Director
Montpellier Cancer Research Institute (IRCM)
Inserm U1194, Montpellier University
+ 33 (0)4 67 61 24 09
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