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Shared habitats

Dangerous drug-resistant superbugs happily co-exist in the gut but can be eliminated by fecal transplants

PLOS

The gut is an important reservoir for drug-resistant bacteria responsible for life-threatening hospital-acquired infections. A study in mice published on September 3rd in PLOS Pathogens reports that two of the most common antibiotic-resistant bacterial species circulating in hospitals occupy and effectively share the same location in the gut, and that they can be eliminated by fecal transplantation of a healthy gut microbiome.

Eric Pamer, from Memorial Sloan-Kettering Cancer Center in New York, USA, and colleagues, investigated the interactions between vancomycin-resistant Enterococcus faecium (VRE) and multi-drug resistant Klebsiella pneumoniae in the intestinal environment. Together, the two pathogens are responsible for about 10% of serious hospital-acquired infections in the US. Both can colonize the gut and spread from there, to the same or other patients, to cause localized or systemic infections.

Using a mouse model of intestinal colonization, the researchers first asked whether intestinal domination by either VRE or K. pneumonia would provide resistance against colonization by the other pathogen. They found that VRE and K. pneumoniae occupy the same intestinal sites and neither compete nor synergize with each other upon dense colonization of the mouse gut. While peacefully co-existing, the researchers found that the two pathogens differ with respect to stimulation and invasion of the colonic mucus, suggesting that they reside in distinct niches that satisfy their specific metabolic needs.

It is known that transplantation of feces from healthy mice can eliminate VRE from the intestine of densely colonized mice and, in humans, fecal transplantation from healthy donors can cure patients with certain pathogenic intestinal infections. To determine whether fecal transplantation can clear K. pneumoniae and concurrent VRE and K. pneumoniae infections, the researchers colonized mice with VRE and K. pneumoniae concurrently and then treated them with fecal microbiota transplants (FMT) or a sterile control solution on three consecutive days.

They found that VRE and K. pneumoniae levels were similar in the feces before FMT administration and remained elevated in mice that received the sterile control solution. In contrast, following FMT treatment, K. pneumoniae density in fecal pellets decreased within one day and became undetectable within 7 days in all mice. VRE, on the other hand, was cleared in 60% of the mice and reduced by a thousand-fold in the remaining 40%.

The researchers conclude that their findings "uncovered previously unrecognized features of VRE and K. pneumoniae colonization and provide insight into the nature of pathogen coexistence, dissemination and ways to eradicate colonization". "Current efforts", they say, "are focused on the identification of commensal bacterial species [i.e. the components of the healthy fecal transplant] that mediate clearance of antibiotic-resistant bacteria".

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Please use this URL to provide readers access to the paper (Link goes live upon article publication): http://dx.plos.org/10.1371/journal.ppat.1005132

Related Image for Press Use: https://www.plos.org/wp-content/uploads/2013/05/Pathogens_Pamer_SEP3_IMG.jpg
Caption: Left, commensal bacteria (red) in the intestine are kept at a distance from the colonic tissue by a thick layer of mucus (green). Antibiotics perturb the integrity of the mucus layer, facilitating invasion by some bacterial species such as Klebsiella pneumoniae (middle) and to a lesser extend VRE (right). Infiltration of the colonic mucus layer may prove to be a critical step in bacterial spread to other sites including the bloodstream.
Credit: Silvia Caballero, CC-BY

Authors and Affiliations:

Silvia Caballero; Memorial Sloan-Kettering Cancer Center, USA; Weill Cornell Graduate School of Medical Sciences, USA
Rebecca Carter; Memorial Sloan-Kettering Cancer Center, USA
Xu Ke; Sloan-Kettering Institute, USA
Boe Sušac; Memorial Sloan-Kettering Cancer Center, USA
Ingrid M. Leiner; Memorial Sloan-Kettering Cancer Center, USA
Grace J. Kim; Memorial Sloan-Kettering Cancer Center, USA
Liza Miller; Memorial Sloan-Kettering Cancer Center, USA
Lilan Ling; Memorial Sloan-Kettering Cancer Center, USA
Katia Manova; Sloan-Kettering Institute, USA
Eric G. Pamer; Memorial Sloan-Kettering Cancer Center, USA; Weill Cornell Graduate School of Medical Sciences, USA

Please contact plospathogens@plos.org if you would like more information.

Funding: This work by supported by grants from the US National Institutes of Health RO1 AI42135 and AI95706, the Tow Foundation and the Lucille Castori Center for Microbes, Inflammation and Cancer to EGP. SC was supported by the Gilliam Pre-doctoral Fellowship from the Howard Hughes Medical Institute. XK and KM were supported by the Core grant P30 CA008748 from the Molecular Cytology Facility at Memorial Sloan-Kettering Cancer Center. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests: The authors have declared that no competing interests exist.

Citation: Caballero S, Carter R, Ke X, Sušac B, Leiner IM, Kim GJ, et al. (2015) Distinct but Spatially Overlapping Intestinal Niches for Vancomycin-Resistant Enterococcus faecium and Carbapenem-Resistant Klebsiella pneumoniae. PLoS Pathog 11(9): e1005132. doi:10.1371/journal.ppat.1005132

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