Public Release: 

Wyss Institute launches Opsonix to commercialize its pathogen-extracting sepsis therapy

The new start-up company is seeking to develop an extracorporeal medical device that removes infectious pathogens and toxins from circulating blood of sepsis patients

Wyss Institute for Biologically Inspired Engineering at Harvard

(BOSTON) - Today the Wyss Institute for Biologically Inspired Engineering at Harvard University unveiled the launch of its start-up company Opsonix Inc. The announcement follows a worldwide exclusive licensing agreement between Harvard's Office of Technology Development (OTD) and Opsonix Inc. enabling the company to ready the Wyss-designed sepsis therapy for clinical use.

Pathogen-induced sepsis strikes at least a million people every year in the U.S. -- more than prostate cancer, breast cancer and AIDS combined-- and over 30% percent of these cases are fatal. There is no therapy specifically approved to treat sepsis and the present standard of care is limited to providing patients with intravenous fluids and broad-spectrum antibiotics, which often does not suffice to halt disease progression. Existing therapies also cannot combat the growing emergence of antibiotic-resistant bacteria, and thus, sepsis represents a huge unmet clinical need.

"We are developing an entirely new approach to treat sepsis that directly and quickly eliminates the pathogens and toxins that trigger the sepsis cascade. Even more importantly, we can accomplish this without having to first identify the infectious agent," said Wyss Institute Founding Director Don Ingber, M.D., Ph.D., who is also the Judah Folkman Professor of Vascular Biology at Harvard Medical School and Boston Children's Hospital and Professor of Bioengineering at the Harvard John A. Paulson School of Engineering and Applied Sciences.

Key to the technology is a genetically engineered protein that is based on a naturally occurring human opsonin protein called Mannose Binding Lectin (MBL). With its ability to bind to a broad spectrum of infectious invaders and endotoxins released by them into the blood stream, MBL usually funnels circulating blood pathogens to the immune system's innate clearance apparatus.

However, to be able to leverage MBL as a blood-cleansing tool, the Wyss team led by Ingber and Michael Super, a Wyss Senior Staff Scientist, fused the pathogen-binding portion of MBL to the Fc region of human immunoglobulin to yield the engineered FcMBL protein.

During the technology's incubation period, for which the Wyss Institute was funded with more than $22 million by the Defense Advanced Research Projects Agency (DARPA) under their Dialysis-Like Therapeutic program, the researchers cleared several obstacles along the preclinical development path.

Putting it to the test in a proof-of-principle study published in Nature Medicine, the team attached FcMBL to nanoscale magnetic beads and demonstrated their ability to effectively clear a wide variety of pathogens and endotoxin from human blood samples when exposed to a magnetic field in a device mimicking the spleen or Biospleen. Moreover, when applied to an extra-corporeal blood circuit attached to septic rats, the Biospleen rapidly cleansed the animals' blood, significantly suppressed their sepsis inflammatory cascades, and prolonged their survival.

"To further de-risk and improve the technology, we had to simplify the device by eliminating the possibility of magnetic beads being carried back into the patients' circulation, and show that the pathogen-extracting method is compatible with current antibiotic therapies," said Super. These challenges now have been successfully addressed in a newly designed device format that was recently published in Biomaterials.

To simplify the therapy for eventual clinical use, the Wyss team attached the pathogen-capturing FcMBL protein to the inner surface of hollow fibers similar to those contained in cartridges already approved by the FDA for dialysis therapy. When tested in rats infected with pathogens or endotoxin, the FcMBL-coated cartridges synergized with commonly used broad-spectrum antibiotics, reducing the infectious pathogen and toxin loads and stabilizing vital signs in the animals more effectively than antibiotics alone. Using FcMBL in cartridges had other important benefits, too: it sped up the pathogen extraction process and considerably reduced the amount of FcMBL protein needed for each individual treatment.

Opsonix has launched with Series A financing led by Baxter Ventures and its founding team includes CEO Eric Devroe, Ph.D., and Scientific Founders Ingber and Super. The company will use the proceeds of the Series A financing to advance the development of the therapy toward clinical investigation.

###

[Movie and Image available]

MULTIMEDIA CONTACT: Seth Kroll, seth.kroll@wyss.harvard.edu

The Wyss Institute for Biologically Inspired Engineering at Harvard University (http://wyss.harvard.edu) uses Nature's design principles to develop bioinspired materials and devices that will transform medicine and create a more sustainable world. Wyss researchers are developing innovative new engineering solutions for healthcare, energy, architecture, robotics, and manufacturing that are translated into commercial products and therapies through collaborations with clinical investigators, corporate alliances, and formation of new start-ups. The Wyss Institute creates transformative technological breakthroughs by engaging in high risk research, and crosses disciplinary and institutional barriers, working as an alliance that includes Harvard's Schools of Medicine, Engineering, Arts & Sciences and Design, and in partnership with Beth Israel Deaconess Medical Center, Brigham and Women's Hospital, Boston Children's Hospital, Dana-Farber Cancer Institute, Massachusetts General Hospital, the University of Massachusetts Medical School, Spaulding Rehabilitation Hospital, Boston University, Tufts University, Charité - Universitätsmedizin Berlin, University of Zurich and Massachusetts Institute of Technology.

Harvard University's Office of Technology Development (OTD) is responsible for all activities pertaining to the evaluation, patenting and licensing of new inventions and discoveries made at Harvard University. Harvard OTD also serves to further the development of Harvard technologies through the establishment of sponsored research collaborations with industry. The mission of Harvard OTD is to promote the public good by fostering innovation and translating new inventions made at Harvard into useful products that are available and beneficial to society. For more information, please visit http://otd.harvard.edu.

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.