Feb. 25, 2016 -- (BRONX, NY) -- Juvenile idiopathic arthritis, or JIA, is the most common form of childhood arthritis. It appears to be an autoimmune disease, caused by antibodies attacking certain proteins in a person's own tissue. But no "autoantigens" -- the proteins triggering an immune attack -- have been linked to JIA.
Now, a new study offers evidence that a human protein called transthyretin (TTR) causes an autoimmune reaction in the joints of JIA patients. The study, led by researchers at Albert Einstein College of Medicine and the Children's Hospital at Montefiore (CHAM), was published online today in the journal JCI Insight.
"Our findings regarding TTR's involvement in JIA point to a potential treatment -- encouraging news for children with this debilitating disease," said study leader Laura Santambrogio, M.D., Ph.D., professor of pathology, of microbiology & immunology, and of orthopaedic surgery at Einstein. JIA patients, she says, might benefit from a drug called tafamidis, which targets TTR. Tafamidis was approved in Europe and Japan for treating familial amyloidosis, which is also linked to TTR. The drug is now undergoing phase III trials in the U.S.
JIA affects about 300,000 children in the U.S. Symptoms include chronic joint pain, swelling and stiffness, which may persist for a few months or a lifetime. There is no cure. Treatments such as nonsteroidal anti-inflammatory drugs (NSAIDs) and biologic response modifiers are used to control symptoms and prevent complications.
In the current study, Dr. Santambrogio and her colleagues looked for abnormal accumulations of proteins in the synovial fluid (which bathes the joints) and blood of patients with JIA. They found a significant increase in TTR in 50 patients at the Children's Hospital at Montefiore, but not in any of the 26 control children who did not have JIA. Further analysis revealed that some JIA patients had unusually high levels of antibodies to the TTR protein. To validate this finding, the researcher analyzed 43 other JIA patients and found a significant increase in TTR autoantibodies in all of them.
TTR is a molecular "chaperone" that transports various molecules, including thyroxine and retinol (vitamin A), in the blood and cerebral spinal fluid. The researchers suspect that JIA begins when TTR collects in the joints.
"The TTR protein has a tendency to misfold and then aggregate, which for some reason seems to occur in children with JIA," said Dr. Santambrogio. "And when proteins aggregate, they tend to become more immunogenic." Using mass spectrometry and other biophysical techniques, the researchers observed misfolded and aggregated TTR in the synovial fluid of JIA patients. The patients' TTR protein was also heavily oxidized, which may further increase its immunogenicity. When abnormal TTR was administered to mice, it elicited a higher immunogenic response (i.e., caused more antibodies to be produced) compared to normal TTR.
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The paper is titled "Autoimmune Response to Transthyretin in Juvenile Idiopathic Arthritis." The other Einstein-Montefiore authors are: Cristina C. Clement, Ph.D., Francesco Bauli, Fawzy A. Saad, Ph.D., Ginger Janow, M.D., Cristina Montagna, Ph.D., Neil Cobelli, M.D., John Hardin, M.D., Norman Ilowite, M.D., and Steven A. Porcelli, M.D. Additional authors include: Halima Moncrieffe, Ph.D., and Aditi Lele of Cincinnati Children's Hospital Medical Center, Cincinnati, OH, Aniuska Becerra and Lawrence J. Stern of University of Massachusetts Medical School, Worcester, MA, and Giorgio Perino, M.D., of Hospital for Special Surgery, New York, NY.
The study was supported by grants from the National Institutes of Health (AG045223, AI38996, and AI48833, AR-47363, NIH AR-48929) and by the Cincinnati Children's Research Foundation and the Cincinnati Genomic Control Cohort. The authors report no conflicts of interest.
About Albert Einstein College of Medicine
Albert Einstein College of Medicine is one of the nation's premier centers for research, medical education and clinical investigation. During the 2015-2016 academic year, Einstein is home to 731 M.D. students, 193 Ph.D. students, 106 students in the combined M.D./Ph.D. program, and 278 postdoctoral research fellows. The College of Medicine has more than 1,900 full-time faculty members located on the main campus and at its clinical affiliates. In 2015, Einstein received $148 million in awards from the National Institutes of Health (NIH). This includes the funding of major research centers at Einstein in aging, intellectual development disorders, diabetes, cancer, clinical and translational research, liver disease, and AIDS. Other areas where the College of Medicine is concentrating its efforts include developmental brain research, neuroscience, cardiac disease, and initiatives to reduce and eliminate ethnic and racial health disparities. Its partnership with Montefiore Medical Center, the University Hospital and academic medical center for Einstein, advances clinical and translational research to accelerate the pace at which new discoveries become the treatments and therapies that benefit patients. Through its extensive affiliation network involving Montefiore, Jacobi Medical Center -- Einstein's founding hospital, and three other hospital systems in the Bronx, Brooklyn and on Long Island, Einstein runs one of the largest residency and fellowship training programs in the medical and dental professions in the United States. For more information, please visit, read our blog, follow us on Twitter, like us on Facebook, and view us on YouTube.
Journal
JCI Insight