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Wolbachia parasite superinfection: A new tool to fight mosquito arbovirus transmission



IMAGE: This is a picture of an A. aegypti, blood feeding. view more

Credit: Cameron P. Simmons

Aedes aegypti mosquitoes transmit a number of pathogens, including the Dengue, Chikungunya, and Zika viruses. Wolbachia, a bacterium that naturally infects many insect species but not Aedes aegypti, can nonetheless be introduced into the Aedes aegypti population and then block virus replication in the infected mosquito host. As with any antiviral strategy, the potential development of resistance by the virus is a concern. A study published on Feb. 18, in PLOS Pathogens reports on a strategy to make it harder for Dengue (and possibly other viruses) to develop Wolbachia resistance.

Wolbachia are vertically transmitted by female mosquitoes to their offspring, and a phenomenon called "cytoplasmic incompatibility" gives infected females a reproductive advantage: they can mate and produce viable (and infected) offspring with infected or uninfected males, whereas the cross between uninfected females and infected males doesn't produce progeny. Over time, these mating outcomes facilitate the replacement of a local, native Aedes aegypti population by a Wolbachia-carrying mosquito population -- and recent field trials in five countries have confirmed this to be the case. Because the infected mosquitoes are resistant to being infected by Dengue, Chikungunya and probably Zika virus, this approach should reduce the number of human cases over time and potentially lead to local elimination of these arboviruses.

Cameron Simmons from the University of Melbourne in Parkville, Australia, and Scott O'Neill, from Monash University in Clayton, Australia, are involved in these field trials. Besides examining the effect on Dengue virus disease in human, they, together with colleagues, are also working on further improvements to the use of Wolbachia in Dengue control. One concern is that the virus will mutate and eventually be able to multiply even in Wolbachia-infected Aedes aegypti hosts. One way to make this harder -- at least in principle -- is to increase the number or type of Wolbachia parasites in the host cells. To this end, the researchers explored the possibility of "superinfection", namely the secondary infection of mosquitoes that already carry one strain of Wolbachia with a second one.

For a second infection to rapidly spread into a population already carrying one Wolbachia strain, it must benefit from cytoplasmic incompatibility, and the second strain must not reduce the fitness of the mosquitoes. And in order to reduce the risk for the development of viral resistance, mosquito hosts that are co-infected must have higher levels of Wolbachia parasites in the host cells that the virus uses for replication.

In this paper, the researchers report the generation of superinfected Aedes aegypti mosquitoes that fulfill these criteria. The first Wolbachia strain is called wMel, and it is the one that is currently spreading through wild Aedes aegypti populations as part of the ongoing field trials. The second strain, called wAlbB, has similar characteristics in singly infected mosquitoes: it grows to relatively high densities in infected mosquitoes and does not diminish their fitness. (A third strain that has also been developed and tested grows to higher densities -- and these are associated with stronger inhibition of virus replication -- but the infected mosquitoes are less fit and therefore do not spread the infection as efficiently).

The researchers found that Aedes aegypti mosquitoes infected with both Wolbachia strains showed normal fitness and had higher parasite densities than mosquitoes infected with either one of the Wolbachia strains alone. Moreover, the two parasite types seemed to populate the same mosquito tissues.

When the researchers compared Dengue virus inhibition in the superinfected mosquitoes with virus in the two singly-infected ones, they found that the superinfected mosquitoes were at least as efficient, and in some tests more, so than singly-infected ones. For example, when the researchers allowed the mosquitoes to feed on human volunteers with a Dengue virus infection, they found that the superinfected mosquitoes had fewer dengue viruses in their salivary glands, suggesting that they might be less likely to transmit the infection to their next human victims.

Critical for its ability to spread into the existing Aedes aegypti population, the double-infected strain showed cytoplasmic incompatibility with either of the single-infected ones, suggesting that it could 'sweep' quickly across areas that has seen previous release of Wolbachia-infected mosquitoes and transform the population.

"In summary", the researchers say, "the generation and characterisation of a superinfected line with the desired phenotypic effects to replace single wild infections provides a potential mechanism to overcome the emergence of DENV resistance. Both Wolbachia strains are stably maintained in the line with minimal mosquito fitness effects. Importantly, DENV replication is inhibited to a greater extent in the superinfected line compared to both parental lines. The observed CI [cytoplasmic incompatibility] phenotype induced by the superinfected line is of particular significance as it would enable the line to be released "on top of" existing wMel or wAlbB field releases in Dengue endemic areas."


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Authors and Affiliations:

D. Albert Joubert, Thomas Walker, Jyotika Taneja De Bruyne, Iñaki Iturbe-Ormaetxe, Scott L. O'Neill School of Biological Sciences, Monash University, Clayton, Melbourne, Victoria, Australia

Lauren B. Carrington, Duong Hue T. Kien, Nhat Le Thanh Hoang, Cameron P. Simmons Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Programme, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam

Lauren B. Carrington, Cameron P. Simmons Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute, Parkville, Melbourne, Victoria, Australia

Nguyen Van Vinh Chau Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam

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Funding: This work was supported by a grant from the Foundation for the National Institutes of Health through the Grand Challenges in Global Health Initiative of the Bill and Melinda Gates Foundation and the National Health & Medical Research Council of Australia programme grant 1037003. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests: The authors have declared that no competing interests exist.

Citation: Joubert DA, Walker T, Carrington LB, De Bruyne JT, Kien DHT, Hoang NLT, et al. (2016) Establishment of a Wolbachia Superinfection in Aedes aegypti Mosquitoes as a Potential Approach for Future Resistance Management. PLoS Pathog 12(2): e1005434. doi:10.1371/journal.ppat.1005434

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