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Skin regeneration in technicolor

Cell Press

Skin regeneration, either after injury or normally to replace dead skin, is difficult to observe at the cellular level. A new system--based on the Brainbow technology that labels individual neurons--genetically color-codes skin cells in zebrafish, allowing researchers to track cell populations in real time. The system, which they call Skinbow, is described March 21 in Developmental Cell.

"One of the barriers to studying regeneration has been to visualize it at high resolution, examining what individual cells are doing and what a large number of individual cells are doing collectively," says senior author Kenneth Poss, a cell biologist at Duke University. "We can catch all of that with this kind of imaging."

Many methods to observe the fate of individual skin cells rely on samples that are "snapshots" of cell growth and movement, which don't tell the whole story. Live imaging of cells is possible but on a small scale. Poss and cell biologist Stefano Di Talia, also at Duke University, wanted to create a system that would allow for real-time imaging of large groups of cells.

The research team, with Duke postdoc Chen-Hui Chen and Alberto Puliafito of the Candiolo Cancer Institute, genetically engineered a line of zebrafish that expressed red, green, and blue fluorescent proteins in different combinations on the uppermost layer of skin cells--even in the epithelium covering the eye.

This skinbow line results in hundreds of potential colors for any given cell to display. At least 70-80 of these colors can be reliably distinguished from one another, says Di Talia, meaning that each cell is unlikely to share a color with its neighbors. The cellular color-coding shows up when the zebrafish are imaged with a microscope under red, green, and blue channels and the images are combined, though the animals have a reddish tint to the naked eye.

"In a very non-invasive manner, we can study single-cell dynamics over a timescale of several weeks," says Di Talia.

The researchers examined the zebrafish under normal conditions, then subjected the study animals to a variety of injuries--ranging from mild alterations like skin exfoliation with a dry tissue to more severe injuries such as fin amputation--to watch how the cells responded. For example, after a zebrafish's fin was amputated, the team observed the following sequence during fin regeneration: first, skin cells rapidly migrated from nearby areas to cover the injury site; second, new epithelial cells were produced to supplement the recruited cells; and third, the cells expanded in size to cover more space at the wound.

"We didn't expect any of this, but with this type of imaging, you don't need to have pre-set ideas or hypotheses," says Poss. "You just need to be able to image and track cells and quantify the data."

The Skinbow system could also be used to study skin cell behavior in different disease models or after drug treatments. "What we have developed in this study is a way to think about, and tools for analyzing, the behavior of individual cells," says Di Talia.


Funding was provided by the National Institutes of Health, the National Science Foundation, and Finalized Research and Founding for Investments in Basic Research.

Developmental Cell, Chen et al.: "Multicolor Cell Barcoding Technology for Long-Term Surveillance of Epithelial Regeneration In Zebrafish"

Developmental Cell (@Dev_Cell), published by Cell Press, is a bimonthly, cross-disciplinary journal that brings together the fields of cell biology and developmental biology. Articles provide new biological insight of cell proliferation, intracellular targeting, cell polarity, membrane traffic, cell migration, stem cell biology, chromatin regulation and function, differentiation, morphogenesis and biomechanics, and regeneration and cellular homeostasis. For more information, please visit To receive Cell Press media alerts, contact

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