Public Release: 

Micro-needle insertion into hippocampus helps brain regeneration in animal model of AD

Mice modeled with Alzheimer's disease undergoing micro-needle procedure had reductions in beta;-amyloid plaques; stimulation of hippocampus also appears to aid in regaining learning abilities

Cell Transplantation Center of Excellence for Aging and Brain Repair

Putnam Valley, NY. (April 26, 2016) - Researchers testing the potential positive effects of "micro-injury" by brief insertion of a small needle into the hippocampal region of mice modeled with Alzheimer's disease (AD) have found that the procedure not only stimulated the hippocampus into regenerative activity, but also reduced β-amyloid plaques, a hallmark of AD.

Their study will be published in a future issue of Cell Transplantation and is currently freely available on-line as an unedited, early epub at:

Micro-needle insertion (acupuncture) has been a mainstay of traditional Chinese medicine for centuries. In the last decade, deep brain stimulation using chronically implanted electrodes into specific brain areas has become common for treating movement disorders such as Parkinson's disease and tremors and paved the way for other micro- needle medical applications.

Because the early and primary damage by AD appears to take place in the hippocampus, an important brain area for learning and memory, the objective for this study was to use micro-needle stimulation in the hippocampus to see if the procedure would both increase hippocampal regeneration and help determine if the needle insertion had an effect on β-amyloid deposits in mice modeled with AD.

"We found that the brief micro needle insertion into the hippocampus of the test animals modeled with AD did prove effective in decreasing amyloid burden," explained Dr. Shijie Song of the Department of Neurology at the University of South Florida in Tampa, FL. "It also improved test animal performance on memory tasks, which indicates possible hippocampal regeneration."

Until recently, many diseases of the central nervous system could not be treated by this method because of inaccessibility of the brain to micro-needles, said the researchers.

"As the cellular and humoral mechanisms of micro-lesioning are better understood, it will be possible to develop novel surgical and pharmaceutical targets for intervention and treatment of neuro-cognitive disorders," concluded the researchers.

"Because Alzheimer's disease is increasing in prevalence, new intervention strategies are becoming invaluable," said Dr. Shinn-Zong Lin, professor of Neurosurgery at China Medical University Hospital in TaiChung, Taiwan and Co-Editor-in-Chief for Cell Transplantation. "Since the host's microenvironment can be inhospitable to transplanted cells and pharmacological interventions in diseased conditions, strategies to increase the regenerative capacity of the patient's own body may be another viable option. Future studies should strive to include a larger sample size in order to validate this approach."


This paper is supported by a VA Merit Grant to S. Song.


Drs. Shijie Song and Juan Sanchez-Ramos
James A Haley Veterans Administration Medical Center and USF Dept. of Neurology 13000 Bruce B. Downs Blvd. Tampa FL 33620
Tel: 813-974-5841

Citation: Song S, Kong X, Sava V, Cao C, Acosta S, Borlongan C, Sanchez-Ramos J. Transient micro-needle insertion into hippocampus triggers neurogenesis and decreases amyloid burden in a mouse model of Alzheimer's disease. Cell Transplant. Appeared or available on-line: February 18, 2016.

The Co-Editors-in-Chief for CELL TRANSPLANTATION are at the Department of Neurosurgery and Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA and the Center for Neuropsychiatry, China Medical University Hospital, TaiChung, Taiwan. Contact: Paul R. Sanberg at, Shinn-Zong Lin at, or Associate Editor Samantha Portis at

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