BOSTON (January 19, 2017) -- Precision medicine - in which diagnosis and treatments are keyed to the genetic susceptibilities of individual cancers - has advanced to the point where it can now impact the care of a majority of children with brain tumors, a new study by investigators at Dana-Farber/Boston Children's Cancer and Blood Disorders Center suggests.
In the largest clinical study to date of genetic abnormalities in pediatric brain tumors, researchers performed clinical testing on more than 200 tumor samples and found that a majority had genetic irregularities that could influence how the disease was diagnosed and/or treated with approved drugs or agents being evaluated in clinical trials. The findings, reported online today by the journal Neuro-Oncology, demonstrate that testing pediatric brain tumor tissue for genetic abnormalities is clinically feasible and that in many cases the results can guide patients' treatment.
The need for new approaches to treating brain cancer in children is urgent, the study authors say. "Although there has been a great deal of progress over the past 30 years in improving survival rates for children with cancer, advances in pediatric brain cancer haven't been as dramatic," says co-lead author Pratiti Bandopadhayay, MBBS, PhD, of Dana-Farber/Boston Children's. "In a recent study, brain tumors accounted for 25 percent of all pediatric deaths attributed to cancer. In addition, many of the current therapies can result in long-term difficulties in cognitive or physical functioning."
Since emerging from research labs more than a decade ago, targeted therapies for cancer have significantly improved the treatment of certain types of leukemia, digestive system tumors, and breast cancer, among other malignancies. The new study is unique in that it reports on the largest collection of pediatric brain tumors to be genetically profiled as patients came to clinic. Pathologists and cytogeneticists performed the testing in a federally approved clinical laboratory--certified under Clinical Laboratory Improvement Amendments (CLIA) as the only type of labs in the United States whose findings can guide patient treatment. Dana-Farber/Boston Children's, the researchers noted, is one of the few centers in the country to regularly analyze the genetics of patients' pediatric brain tumors.
The researchers plumbed the genomes of 203 pediatric brain tumor samples, representing all major subtypes of the disease. They analyzed 117 of the samples with OncoPanel testing, a technology that sequences the exomes - the sections of DNA that hold the blueprints for making specific cell proteins --- for irregularities in 300 cancer-related genes. They also studied 146 samples tested with OncoCopy, which examines how many copies of genes are missing or overabundant within the tumor cells. Sixty samples underwent both forms of testing ,which allowed researchers to explore whether combining the two tests was more powerful than each alone.
Of the samples tested by OncoPanel, 56 percent harbored genetic abnormalities that were clinically relevant - that could impact a patient's diagnosis or be targeted by drugs already in clinical use or under study in clinical trials. (Many of these drugs cross the blood-brain barrier, the dense web of cells that can prevent medicines from exiting the bloodstream to reach the brain.) Among the findings:
- Alterations were found in the gene BRAF, one of the most commonly mutated genes in pediatric brain tumors, and one for which several targeted drugs are being tested.
- The two-pronged testing approach revealed clinically relevant abnormalities in 89 percent of medulloblastomas, which account for nearly a fifth of all brain tumors in children. Combining the two tests was found to be particularly useful for these patients.
"The importance of genomic profiling in the diagnosis and treatment of pediatric brain cancers is reflected in the World Health Organization's recent decision to classify such tumors by the genetic alterations within them, rather than by broad tumor type" says study co-senior author Susan Chi, MD, of Dana-Farber/Boston Children's. "Targeted therapies are likely to be most effective when they're matched to specific abnormalities within tumor cells. Our findings show that precision medicine for pediatric brain tumors can now be a reality."
The co-lead authors, with Bandopadhayay, of the study are: Shakti Ramkissoon of Dana-Farber Cancer Institute and Brigham and Women's Hospital, Jaeho Hwang, of Harvard Medical School, and Lori Ramkissoon, PhD, of Dana-Farber. Co-senior authors, with Chi, are Rameen Beroukhim, MD, PhD, of Dana-Farber, Brigham and Women's, and the Broad Institute of MIT and Harvard, and Keith Ligon, MD, PhD, of Dana-Farber/Boston Children's, Brigham and Women's and the Broad Institute.
The co-authors are Hayley Malkin, Mariella Filbin, MD, PhD, Ashley Plant, MD, Michael Chang, MD, MS, Edward Yang, Karen Wright, MD, Peter Manley, MD, Sanda Alexandrescu, MD, Alanna Church, Katherine Janeway, MD, Marian Harris, MD, and Mark Kieran, MD, PhD, of Dana-Farber/Children's; Liliana Goumnerova, MD, of Boston Children's Hospital; Noah Greenwald, Ryan O'Rourke, Nathan Pinches, Claire Sinai, Matthew Ducar, and Charles Stiles, PhD, of Dana-Farber; Steven Schumacher, MS, of Dana-Farber and the Broad Institute; Wenya Bi, MD, Laura MacConaill, PhD, Rebecca Folkerth, Azra Ligon, PhD, and Sandro Santagata, MD, PhD, of Dana-Farber and Brigham and Women's; Hart Lidov, MD, Ivana Delalle, MD, PhD, Neal Lindeman, MD, and Adrian Dubuc, PhD, of Brigham and Women's.
Funding for the study was provided by: The PLGA Foundation; A Kids' Brain Tumor Cure Foundation: Stop and Shop Pediatric Brain Tumor Program; the Path to Cure Foundation; St Baldrick's Foundation; Team Jack Foundation; the Pediatric Brain Tumor Foundation; the Andrysiak Fund for LGG; Jared Branfman Sunflowers For Life Fund For Pediatric Brain And Spinal Cancer Research; the Sontag Foundation ; the National Institutes of Health (grant numbers P50CA165962, R01 CA188228, P01 CA142536, CA165962, and K08NS087118-03), Ian's Friends Foundation, Alex's Lemonade Stand Foundation, the Gilmore Fund, Cure ATRT Now, the Hamilton Low-Grade Glioma Fund, the Joe Andruzzi Fund, the Itzkovitz Low-Grade Glioma Fund, the Olivia Caldwell Foundation, and Lauren's First and Goal Foundation.
About Dana-Farber/Boston Children's Cancer and Blood Disorders Center
About Dana-Farber/Boston Children's Cancer and Blood Disorders Center
Dana-Farber/Boston Children's Cancer and Blood Disorders Center -- the nation's top pediatric cancer center, according to U.S. News & World Report - brings together two internationally known research and teaching institutions that have provided comprehensive care for pediatric oncology and hematology patients since 1947. The Harvard Medical School affiliates share a clinical staff that delivers inpatient care at Boston Children's Hospital and most outpatient care at Dana-Farber Cancer Institute.