Amyotrophic Lateral Sclerosis, commonly known as ALS, is an incurable neurodegenerative disease that affects all voluntary muscles in the body leading to paralysis and breathing difficulties. Eye muscles, in contrast to other muscles, generally retain their mobility even in the final stages of the disease.
A dissertation at Umeå University in Sweden has studied the two mechanisms that could be of importance to the eye muscles' retained function in ALS patients. The research also attempts to provide clues to novel treatment methods based upon these findings.
Eye muscles are very specialised and differ greatly from other muscles in several areas, for instance in their neuromuscular junctions formed between muscle fibres and nerve cells. Previous research has suggested that ALS causes a break-down starting in the nerve connection and spreading inwards to nerve cell bodies in the brain and spinal cord.
"We could see that the nerve connection to the eye muscles remains intact even in stages of the disease where a large proportion of neuromuscular junctions had been lost in leg muscles," says Anton Tjust, doctoral student at the Department of Integrative Medical Biology and the Department of Clinical Sciences at Umeå University, and author of the dissertation.
"The loss of connection between nerves and muscles can be seen in rather early stages of the degenerative process. We can hence establish that eye muscle protection mechanisms are active at a relatively early stage of the disease. Our results do not, however, ascertain if the disease starts in the nerve connection or in the cell body."
A previous study, by the research team that Anton Tjust is a part of, showed that certain fibre types present in the eye muscles seem to decrease in number in ALS. In the dissertation, it is confirmed that some muscle fibre types in the eye muscles show changes in the final stages of the disease. One of these specialised fibre types, so-called multiply-innervated muscle fibres, decreased by up to a third in certain patients.
"It seems like certain muscle fibre types in the eye muscles are not at all resilient to ALS, it is rather other fibre types in the eye muscles that preserve their functions so well. However, pinpointing these particular fibre types still remains to be done," says Anton Tjust.
The researchers have also investigated muscle stem cells in eye muscles and other muscle types. Muscle stem cells in the eye muscles have been suggested to be more efficient than muscle stem cells in arm and leg muscles. The researchers aimed to answer if muscle stem cells in eye muscles can contribute to its preservation despite ALS, and whether these cells decrease in number in arm and leg muscles at advanced stages of ALS.
"We found that the number of muscle stem cells in the eye muscles didn't differ greatly between healthy controls and ALS patients. Furthermore, the number of muscle stem cells in the eye muscles were relatively moderate, which is the opposite of what has previously been declared. However, an accumulation of muscle stem cells in the front part of the eye muscles could be seen, which could explain the current impression that eye muscles are rich in muscle stem cells," says Anton Tjust.
The research team measured the number of activated and maturing muscle stem cells in arm and leg muscles in deceased ALS patients, as well as in age-matched controls. The result showed that muscles in patients where the ALS had reached very advanced stages had a normal or even increased amount of muscle stem cells in various stages of activation and maturity. Nevertheless, only moderate levels of muscle stem cells were seen in the eye muscles of both healthy donors and deceased ALS patients. Based upon these findings, the researchers draw the conclusion that these stem cells do not wear out as the disease progresses in leg and arm muscles and have a very limited role in the retained eye muscle function in ALS patients.
"The fact that the eye muscles actually retain their function so well in ALS patients suggests that there nevertheless is some form of protective mechanism. Since ALS is a death sentence for patients and a tragedy to the entire family, we as researchers have an obligation to continue conducting research until we have an efficient treatment to offer," says Anton Tjust.
Umeå University is located in the north of Sweden characterised by strong research conducted in a vast number of fields, and many of our researchers belong to the global elite in for instance global health, epidemiology, molecular biology, ecology, plant physiology and Arctic research. Umeå University is one of Sweden's largest teaching universities that offers a wide-spanning and attractive selection of courses and programmes, and stimulating environments for working and studying for the over 4,300 employees and 31,000 students. It was from Umeå University that the work in discovering the gene-editing tool CRISPR-Cas9 was led.