image: Working model for the cross-kingdom transfer of plant microRNAs in the regulation of honeybee development. For larvae that are destined to become queens, royal jelly is fed in copious amounts to drive the development of the royal phenotype. For worker-destined larvae, substantial quantities of plant microRNAs are absorbed when consuming beebread and pollen, which can negatively influence the development of the royal phenotype in honeybees by regulating multiple genes in caste differentiation pathways. view more
Credit: Drs. Xi Chen and Kegan Zhu
Bee larvae develop into workers, in part, because their diet of pollen and honey, called beebread, is rich in plant regulatory molecules called microRNAs, which delay development and keep their ovaries inactive. Xi Chen of Nanjing University in China and colleagues, report these August 31, 2017 in PLOS Genetics.
Researchers have long known that diet plays an important role in the complex process that determines whether a honeybee larva will become a worker or a queen. While the workers primarily consume beebread, the queens feast on royal jelly secreted by the glands of nurse bees. Beebread contains much higher levels of plant microRNAs than royal jelly, so researchers decided to investigate if these molecules, which regulate gene expression in plants, could also impact honeybee caste development. They found that honeybees raised in the lab on simulated beebread supplemented with plant microRNAs developed more slowly and had a smaller body and smaller ovaries than larvae raised without the supplements. The plant microRNAs also had a similar effect on fruit fly larvae, even though fruit flies are not social insects. Further experiments showed that one of the most common plant microRNAs in beebread targets the TOR gene in honeybees, which helps determine caste.
The study shows that there is more to the story of honeybee caste formation than the traditional focus on royal jelly and identifies a previously unknown function of plant microRNAs in fine-tuning larval development. The work is a powerful example of the effects of cross-kingdom microRNAs and how these interactions can affect a species' development and evolution.
"Regulation of honeybee development by plant microRNAs shows an evolutionary adaptation for colony success through partnership between two species. Further studies in this new field may shed light on the impact of food processing on the evolution of eusociality" said Dr. Xi Chen.
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In your coverage please use this URL to provide access to the freely available article in PLOS Genetics: http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1006946
Citation: Zhu K, Liu M, Fu Z, Zhou Z, Kong Y, Liang H, et al. (2017) Plant microRNAs in larval food regulate honeybee caste development. PLoS Genet 13(8): e1006946. https://doi.org/10.1371/journal.pgen.1006946
Funding: This work was supported by: the National Basic Research Program of China (973 Program) (No. 2014CB542300) http://program.most.gov.cn/; the National Natural Science Foundation of China (Nos. 31271378 and 81250044) http://www.nsfc.gov.cn/; the Research Special Fund for Public Welfare Industry of Health (No. 201302018). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing Interests: The authors have declared that no competing interests exist.
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PLoS Genetics