Multiple laboratories have observed unusual neural activity resembling epilepsy in some lines of genetically modified mice widely used in neuroscience research. Reporting their findings in eNeuro, the authors caution that this activity is easy to miss and presents potential challenges for using these animals to study the healthy brain.
Calcium imaging is a common technique for measuring the activity of single neurons. Various mouse lines have been developed to express proteins that can be used to visualize changes in concentrations of calcium, which increase when neurons become active.
Nicholas Steinmetz and colleagues found that some mice expressing the genetically-encoded calcium sensor GCaMP6 display brief bursts of electrical activity, commonly observed in epileptic patients and animal models. Using a drug to suppress expression of GCaMP6 until the mice were 7-weeks-old prevented these events, suggesting that expression of this sensor during development may give rise to the activity they observe. The authors note that, apart from rare seizures, the mice's behavior is not affected in obvious ways and this activity is not observed in the commonly studied visual cortex, which can make it difficult to detect. They advise other researchers to confirm observations in these mice with multiple techniques and describe alternative mouse lines that may be used instead.
Article: Aberrant Cortical Activity In Multiple GCaMP6-Expressing Transgenic Mouse Lines
Corresponding author: Nicholas Steinmetz (University College London, UK), firstname.lastname@example.org
eNeuro is an online, open-access journal published by the Society for Neuroscience. Established in 2014, eNeuro publishes a wide variety of content, including research articles, short reports, reviews, commentaries and opinions.
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The Society for Neuroscience is the world's largest organization of scientists and physicians devoted to understanding the brain and nervous system. The nonprofit organization, founded in 1969, now has nearly 37,000 members in more than 90 countries and over 130 chapters worldwide.