Researchers have demonstrated for the first time that extracellular vesicles - tiny protein-filled structures - isolated from amniotic fluid stem cells (AFSCs) can be used to effectively slow the progression of kidney damage in mice with a type of chronic kidney disease. The findings, by a research team at the Saban Research Institute of Children's Hospital Los Angeles, provide new insights about the mechanisms of kidney disease and point to a new approach for improved treatments. Results of the study were recently published online in Scientific Reports.
Alport syndrome, a type of chronic kidney disease, is an inherited disease that causes damage to the kidneys by the progressive damage to the glomerulus - the area of the kidney responsible for filtering blood. In patients with Alport syndrome, vascular endothelial growth factor (VEGF) - is known to be elevated early on in the disease. Increased VEGF causes scar tissue and severe damage to the cells that line the surface of blood vessels in the glomeruli eventually leading to kidney failure.
"By the time children born with Alport syndrome reach adult life, they typically need dialysis to clear the waste from their blood and will require a kidney transplant," said Laura Perin, PhD, co-director of the GOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics in Urology and principal investigator of the study.
In earlier studies, Perin's research team demonstrated that injection of AFSCs could delay the progression of renal fibrosis and glomerular sclerosis in particular in mice with Alport syndrome. However, the mechanisms responsible for this therapeutic effect had not been determined.
In the current study, the research team hypothesized that AFSCs provide this protection to the kidney through release of extracellular vesicles-tiny membrane surrounded structures that contain receptors on their surface that can bind VEGF and reduce its biological activity on renal cells. The researchers isolated these vesicles and found that they could effectively target the glomerulus to control cellular homeostasis and maintain a stable level of functioning.
"We have demonstrated that these vesicles can be used to regulate VEGF activity and prevent the glomerular capillary damage," said Sargis Sedrakyan, PhD, who is the first author on the paper. "We can efficiently use the vesicles to help restore normal kidney function by curbing the progression of endothelial damage in the filtration unit of the kidney."
While vesicles previously have been shown to be promising targets for developing treatments, this study represents the first time they have been applied to glomerular disease by preventing endothelial damage. The finding represents a potential glomerulus-targeted intervention for chronic kidney disease.
"The advantage of this approach is that we can really modify the amount and the types of extracellular vesicles that we inject and they seem to be homing to and acting directly in the area that requires treatment," explained Perin.
The team's next step will be to validate the stem cell-derived vesicle in different types of kidney disease with the final aim of finding a therapy that is effective for all patients who suffer from chronic kidney disease.
This project was conducted in collaboration with B. Bussolati from the University of Torino, Italy and additional contributors that include, V. Villani, S. Da Sacco, N. Tripuraneni, A. Achena, M. Lavarreda-Pearce, A. Petrosyan, H. Soloyan, R. E. De Filippo, all of Children's Hospital Los Angeles, along with S. Porta of the Department of Molecular Biotechnology and Health Sciences at the University of Torino, Italy. The research was supported by the GOFARR Fund at Children's Hospital Los Angeles, the Post-Doctoral Fellowship from the California Institute for Regenerative Medicine and the Research Career Development Fellowship from the Saban Research Institute of CHLA.
About Children's Hospital Los Angeles
Children's Hospital Los Angeles has been ranked the top children's hospital in California and sixth in the nation for clinical excellence with its selection to the prestigious U.S. News & World Report Honor Roll. CHLA is home to The Saban Research Institute, one of the largest and most productive pediatric research facilities in the United States. Children's Hospital is also one of America's premier teaching hospitals through its affiliation with the Keck School of Medicine of the University of Southern California since 1932. For more information, visit CHLA.org. Follow us on Twitter, Facebook, YouTube, LinkedIn and Instagram, and visit our child health blog (CHLA.org/blog) and our research blog (ResearCHLABlog.org).