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The bacterial 'Game of Thrones'

Understanding bacterial wargames inside our body

University of Oxford

IMAGE

IMAGE: Two bacterial colonies fighting on solid media using toxins. Upon detecting an incoming attack from the red strain, cells of the bottom strain pass this information on to others in... view more 

Credit: Kevin Foster

Much like animals and to a degree humans, bacteria enjoy a good fight. They stab, shove and poison each other in pursuit of the best territory. While this much is clear, little is known about the tactics and strategy that bacteria use during their miniature wargames.

In a study published in Current Biology, researchers at the University of Oxford have shed light on this area of bacterial behaviour, revealing that bacteria approach conflict in much the same way as an army by responding to a threat with a coordinated, collective retaliation.

The team studied pairs of Escherichia coli strains as they fought against each other. Each strain uses a particular toxin to try to overcome its competitor. A strain is immune to its own toxins, but it can kill other strains. This type of competitive interaction plays a key role in how individual bacteria establish themselves in a community, such as the human gut. By engineering the strains to have a fluorescent-green colour, the authors were able to clearly follow their combat in real time.

The findings revealed that not all strains of bacteria fight the same way. Each approaches conflict with a different level of attack, some being hyper-aggressive and others much more passive. In addition to these basic differences in aggression, the research also shows that some strains can not only detect an attack from an incoming toxin, but they can also respond quickly to warn the rest of the colony. Cells on the edge of the colony will detect the incoming attack, and share this information with the cells behind the battlefront, allowing them to respond as a collective, in a coordinated and surprisingly sophisticated fashion.

While these phenomena are well known in animals, the study is a first of its kind for observing this behaviour in bacteria. Professor Kevin Foster, senior author on the work and Professor of Evolutionary Biology in the Department of Zoology at the University of Oxford, said: 'Our research shows that what appear to be simple organisms can function in a very sophisticated manner. Their behaviour is more complex than we have previously given them credit for. Much like social insects, such as honey bees and wasps and social animals like birds and mammals who use alarm calls, when under predation, they are capable of generating a coordinated attack'.

Since the human body plays host to vast numbers of bacteria, particularly our gut microbiome, this effectively means that there is a bacterial war going on inside us. Understanding bacterial competition can help us to understand how bacteria spread, where and why. Professor Foster explains: 'We know from other studies that toxins are important for whether or not a particular strain will establish in a community. But understanding how bacteria release toxins and out-compete others is very important for understanding the spread of infection.'

The team are in the process of building on this work to understand how bacteria can use toxins to provoke and misdirect aggression in their opponents. Dr Despoina Mavridou, one of the lead authors on the study, said: 'Warfare based on provocation can be beneficial. It is most likely taking place in the gut, where bacteria may provoke multiple opponents to attack and wipe out each other.'

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Notes to editors:

The paper will appear online at this link when the embargo lifts: http://www.cell.com/current-biology/fulltext/S0960-9822(17)31663-9 and the DOI will be: 10.1016/j.cub.2017.12.030

The study's other authors are Dr Diego Gonzalez Molecular Biologist at Oxford's Foster Lab in the Department of Zoology (lead author), Dr Wook Kim, Assistant Professor at Duquesne University and Professor Stuart West, Professor of Evolutionary Biology at Oxford's Department of Zoology.

For further information please contact Lanisha Butterfield, Media Relations Manager at Oxford University, on 01865 280531 or email lanisha.butterfield@admin.ox.ac.uk

FIGURES

Fig1. Two bacterial colonies fighting on solid media using toxins. Upon detecting an incoming attack from the red strain, cells of the bottom strain pass this information on to others in the colony leading to a massive collective attack against the red strain. Green colour indicates toxin production.

Video showing two strains fighting: https://www.youtube.com/watch?v=pg6WUDn16Us&feature=youtu.be

Two bacterial colonies fighting on solid media using toxins. These strains can detect the attack of the other, and pass the information on within their colony, to launch massive coordinated attacks on each other. This results in a "no man's land" in between the two strains where cells are killed (with the exception of a few resistant cells that grow into new little colonies). Strains are fluorescently labelled and they turn green when they are releasing toxins. Video edited with the help of Dr Patricia Bernal, Imperial College London.

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