Quebec City, Feb. 1st, 2018 -- Platelets play a much more important role in our immune system than previously thought, according to a study published by researchers from Université Laval and Centre hospitalier universitaire (CHU) de Québec Research Centre. In addition to their role in coagulation and healing, platelets also act as the immune system's first responders when a virus, bacterium, or allergen enters the bloodstream. This discovery, details of which have been published in the Proceedings of the National Academy of Sciences (PNAS), opens the door to new ways to treat patients with septic shock caused by viral or bacterial infection as well as people with auto-immune diseases like rheumatoid arthritis and lupus.
When a foreign body enters the blood for the first time, it causes the formation of antibodies, explained Éric Boilard, lead author and professor at the Université Laval Faculty of Medicine. The next time these antibodies encounter the same foreign body, they quickly attach to its surface to form antigen-antibody complexes that trigger an inflammatory response.
Platelets have receptors that recognize these complexes, and this is what led Professor Boilard and his research partners to suspect that platelets were involved in the inflammatory process. To test their hypothesis, they created antigen-antibody complexes in the blood of mice using a virus, a bacterial toxin, and an allergenic protein.
The results were similar in all three cases. The mice showed the classic symptoms of septic or anaphylactic shock, namely, a drop in body temperature, tremors, impaired cardiac function, vasodilation, and loss of consciousness. "We repeated the tests on mice with almost all platelets removed and on mice with no antigen-antibody complex receptors on their platelets. These mice had no physiological response, which clearly demonstrates the key role of platelets in the process. Platelets, and not white blood cells, are first on the scene during an immune response," said the professor.
The researchers established that the mice went into shock because the platelets had released serotonin. "It's the same molecule as the neurotransmitter in the brain, but the molecule in the platelets is produced by cells in the intestine. Platelets store serotonin--they contain 90% of the body's entire serotonin supply--and release it in certain situations," Boilard explained.
One of the study's clinical implications is that platelet transfusion for patients in septic or anaphylactic shock could aggravate their condition by increasing the amount of serotonin in the blood. "Transfusion remains important, especially since those patients often show low platelet levels, but in order to prevent the problem the antigen-antibody complex receptors on the platelets should be blocked before transfusion," Boilard said. He is now researching the role of the antigen-antibody complex receptor in auto-immune diseases like arthritis and lupus. "We believe that by blocking the receptor, we should be able to improve a patient's condition without affecting everything else that platelets do," he noted.
The study is signed by 24 researchers, 14 of whom are from Université Laval and Centre de recherche du CHU de Québec-Université Laval: Nathalie Cloutier, Isabelle Allaeys, Geneviève Marcoux, Benoit Mailhot, Anne Zufferey, Tania Lévesque, Yann Becker, Nicolas Tessandier, Imene Melki, Guy Poirier, Louis Flamand, Steve Lacroix, Paul Fortin, and Éric Boilard.
Faculty of Medicine
Centre hospitalier universitaire (CHU) de Québec Research Centre
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