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Helmintex assay detects low intensity schistosomiasis infections

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Credit: Graeff-Teixeira, et al, 2018

Individuals who release low numbers of schistosome eggs in their feces may not be detected by classical methods to detect schistosomiasis. Instead, the Helmintex method-- which uses a magnetic field to isolate eggs--is more sensitive for these low intensity schistosomiasis infections, researchers report in PLOS Neglected Tropical Diseases.

Schistosomiasis is a common infection that affects over 290 million individuals, especially in Sub-Saharan Africa, Asia and South America. Classical diagnostic methods, including the Kato-Katz (KK) fecal smear recommended by the World Health Organziation, are not accurace in situations where low egg burden is present in stool. Alternative methods, including a point-of-care immunodiagnostic for schistosome cathodic circulating antigen in urine (POC-CCA) and the Helmintex (HTX) method, have been proposed as substitutes.

In the new work, Carlos Graeff-Teixeira of the Pontificia Universidade Catolica de Rio Grande de Sul, and colleagues compared the KK, POC-CCA and HTX methods in a population-based study. 461 children and adults from Candeal, Brazil provided stool, urine, and blood samples for the comparison; an additional 220 submitted at least one specimen type.

The prevalence of schistosomiasis was found to be 71% with the POC-CCA method, 40% with HTX, and 11% with KK. More than 70% of individuals had very low egg burdens in their feces. However, the POC-CCA method provided the lowest proportion of true positive results. The HTX method was 100% sensitive with egg burdens higher than 1.3 epg and detected eggs in 29% of the samples that were negative according to the KK method.

"The HTX method is very sensitive and should be used as a reference method for diagnosing intestinal schistosomiasis and for comparative evaluation of other tests," the researchers say. "The HTX method should also be considered for use in the monitoring and certification of transmission interruption."

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In your coverage please use this URL to provide access to the freely available article in PLOS Neglected Tropical Diseases: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006274

Citation: Lindholz CG, Favero V, Verissimo CdM, Candido RRF, de Souza RP, dos Santos RR, et al. (2018) Study of diagnostic accuracy of Helmintex, Kato-Katz, and POC-CCA methods for diagnosing intestinal schistosomiasis in Candeal, a low intensity transmission area in northeastern Brazil. PLoS Negl Trop Dis 12(3): e0006274. https://doi.org/10.1371/journal.pntd.0006274

Funding: Financial support (PVE 401904/2013-0) was provided by Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico do Brasil (CNPq: http://www.cnpq.br), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES: http://www.capes.gov.br) "Auxílio-PE-Parasitologia 1427/2011/Edital 32". C. Graeff-Teixeira is a recipient of a CNPq PQ 1D fellowship and grants (307005/2014-3). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests: The authors of this manuscript have the following competing interests: Carlos Graeff-Teixeira has a patent deposited in Brazil for the method Helmintex.

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