BOSTON, Aug. 20, 2018 -- America's drug problem may be even worse than officials realize. And illicit drugs are consumed at a higher rate during celebratory events. Those are just two of the conclusions scientists have drawn from recent studies of drug residues in sewage.
The researchers will present their findings today at the 256th National Meeting & Exposition of the American Chemical Society (ACS). ACS, the world's largest scientific society, is holding the meeting here through Thursday. It features more than 10,000 presentations on a wide range of science topics.
In the U.S., more than 28 million people aged 12 or older used an illicit drug in 2016, including 12 million who misused opioids, according to estimates from the U.S. Department of Health and Human Services (HHS). Collecting such data has some challenges, however.
"The conventional approach to assess community drug usage in the U.S. takes months or years," Bikram Subedi, Ph.D., says. It's also costly, and researchers have had to rely on drug-related crime statistics, overdose/toxicology reports and the public's responses to surveys, which could well underreport use, he adds.
As a faster, inexpensive alternative, Subedi's team at Murray State University uses "sewage epidemiology," a technique for analyzing wastewater. Subedi, the project's lead investigator, says this method gleans nearly real-time data on drug consumption in local communities. The testing shows that consumption of some drugs is far more widespread than HHS estimates suggest. It also confirms the intuitive assumption that drug use rises during celebrations.
In a prior study, Subedi analyzed drug use in New York. His recent studies were carried out in Kentucky, where undergraduate Katelyn Foppe and others in Subedi's lab assessed whether special events, such as the Fourth of July or the 2017 solar eclipse, would impact drug use in two towns. They also wanted to know if the effects would differ in the towns, which are nearly the same size, just 50 miles apart and governed by the same rules and regulations.
The researchers collected samples at each town's sewage treatment plant. After returning to the lab, the team filtered and extracted the samples and then analyzed them with high-performance liquid chromatography and mass spectrometry. "The results showed that consumption of drugs like methamphetamine, cocaine and THC -- the main active ingredient in marijuana -- was significantly higher during festive events," Subedi says. "But the profile and rate of consumption was significantly different in the two towns." He plans to do further studies to explore the reasons for that difference.
Calculations based on the tests revealed levels of methamphetamine consumption that are among the highest ever reported in the U.S. In addition, the percentage of the population using amphetamine and methamphetamine was two- to four-fold higher than HHS estimates, according to the researchers. The samples also showed very high consumption of opiates such as hydrocodone, oxycodone, Percocet and morphine, Subedi says. He notes that Kentucky is in a region infested with illegal meth labs and burdened with extremely high opiate prescription rates.
Although sewage epidemiology is widely applied in Europe, it has been underused in the U.S., Subedi notes. He uses a state-of-the-art analytical method -- isotope dilution mass spectrometry -- that incorporates internal standards, or isotopically labeled versions of drug molecules in which selective hydrogen atom(s) are replaced with heavier deuterium. Adding a small amount of these labeled molecules during an analysis allows the researchers to confirm the identity and concentration of drugs they suspect are in a given wastewater sample.
Subedi's current sample-collection method can't zero in on the specific sites of illicit drug use within a community. So the team plans to move upstream, collecting sewage samples from the suspected individual sites before they're commingled at the wastewater treatment facility. This refinement would reveal whether drug use is homogeneous throughout a particular town, or if there are hotspots of consumption, such as a particular neighborhood, hospital or school. Subedi also wants to track population data so he can correct for an influx of tourists during a festive event, for example, and to expand sewage testing nationwide.
A press conference on this topic will be held Wednesday, Aug. 22, at 9:30 a.m. Eastern time in the Boston Convention & Exhibition Center. Reporters may check-in at the press center, Room 102A, or watch live on YouTube https:/
The research was funded by the National Institute of General Medical Sciences through the Kentucky Biomedical Research Infrastructure Network.
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Estimation of the consumption of illicit drugs during special events in two communities in Western Kentucky, USA using sewage epidemiology
Sewage epidemiology is a cost-effective, comprehensive, and a non-invasive technique capable of determining semi-real-time community usage of drugs utilizing the concentration of drug residues in wastewater, wastewater inflow, and the population served by a wastewater treatment plant. In this study, semi-real-time consumption rates of ten illicit drugs were determined using sewage epidemiology during special events including Independence Day, the 2017 solar eclipse, and the first week of an academic semester in the Midwestern United States. The average per-capita consumption rate of amphetamine, methamphetamine, cocaine, and THC were significantly different between two similar-sized communities during Independence Day observation week (p <0.046) and a typical week (p <0.001). Compared to a typical day, the consumption rate of amphetamine, methamphetamine, cocaine, morphine, and methadone was significantly higher on Independence Day (p <0.021) and during solar eclipse observation (p = 0.020). The estimated percentage of the population that consumed cocaine in a community is similar to the conventionally estimated consumption of cocaine; however, the combined estimated population that consumed amphetamine and methamphetamine based on sewage epidemiology was ~2 to 4 fold higher than the conventional estimates. This study is the first to compare community use of drugs during special events in the USA using sewage epidemiology.
Are illicit drugs consumed more during celebrations?
Conventionally, the consumption rate of drugs in a community is determined based on self-reported surveys, overdose/toxicological reports, and drug-related crime statistics. Cost-intensive conventional methods are, therefore, prone to underestimate community use of drugs. Drug's residues in wastewater were utilized - Sewage Epidemiology - to determine the consumption rate of drugs during special events in two communities in Western Kentucky, USA. Consumption of amphetamine, methamphetamine, cocaine, morphine, and methadone on July 4th were significantly higher (p < 0.021) than in July 3rd and 5th in both communities. Similarly, the per-capita consumption of amphetamine, methamphetamine, cocaine, morphine, methadone, and THC on the 2017 solar eclipse observation day was significantly higher than a normal Monday. The percentage of the population which consumed amphetamine and methamphetamine in this study is up to two orders of magnitude higher than SAMSHA's estimations. While the opioid epidemic has been declared as a national public health emergency in the USA, codeine and hydrocodone were the most consumed prescription opioids. The discharged wastewater was also found to be a source of several neuropsychiatric and illicit drugs into the receiving creek and river.