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The FASEB Journal: Gut microbiota regulated by hydrogen peroxide in lactation

Federation of American Societies for Experimental Biology

Gut microbiota of mouse pups regulated by hydrogen peroxide produced through lactation

A recent animal study published in The FASEB Journal explores the uncharted territory of how the gut microbiome develops in infancy. It was well known that breast-fed mouse pups have a simple bacterial population dominated by the health-promoting Lactobacillus bacteria. But the molecular mechanism of how mouse mother's milk suppresses a more diverse gut microbiome has been unclear.

Previously, researchers examined the role of L-amino acid oxidase 1 (LAO1) -- an enzyme that converts L-amino acids into keto acids, ammonia, and hydrogen peroxide (H2O2) -- in mouse mothers' milk. That study demonstrated that the H2O2 in milk prevented the mother's mammary gland from developing bacterial infectious disease, such as mastitis.

The same group of researchers conducted a more recent mouse study to consider whether the H2O2 generated by LAO1 might impact the development of infant gut microbiota via breast-feeding. Indeed, they found that LAO1 suppressed microbial diversity during the pups' gut microbiota development.

To conduct the study, researchers examined the amount of Lactobacillus in the microbiota of two groups of breast-fed infant mice: a control group and a group with a deleted (or "knockout") LAO1 gene. As expected, the mice in the control group displayed Lactobacillus-dominated microbiota. The microbiota of the LAO1-knockout mice, however, displayed lower levels of Lactobacillus. The study suggests for the first time that H2O2 acts as a gatekeeper for bacterial selection and the development of gut microbiota during lactation in mice.

"While there are many differences between human and mouse mothers' milk, both types of neonates have a low-diversity microbiota, which might be important to reducing their risk of disease in adulthood," said Kentaro Nagaoka, PhD, an associate professor at the Laboratory of Veterinary Physiology within the Tokyo University of Agriculture and Technology's Department of Veterinary Medicine. "There is a need for further research exploring how we can control breast milk compounds to ensure infants inherit the good microbiome."

"This was a clever and much-needed experiment, and the results have important human impacts," said Thoru Pederson, PhD, Editor-in-Chief of The FASEB Journal.


This research was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.

The FASEB Journal is published by the Federation of the American Societies for Experimental Biology (FASEB). The world's most cited biology journal according to the Institute for Scientific Information, it has been recognized by the Special Libraries Association as one of the top 100 most influential biomedical journals of the past century. Receive monthly highlights for The FASEB Journal; subscribe at

FASEB is composed of 30 societies with more than 130,000 members, making it the largest coalition of biomedical research associations in the United States. Our mission is to advance health and well-being by promoting research and education in biological and biomedical sciences through collaborative advocacy and service to our societies and their members.

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