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Genetic study reveals how citrus became the Med's favorite squeeze

John Innes Centre

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IMAGE: Buddha's Hands -- citron study highlights historic path of domestication. view more 

Credit: Mathulack Photography

Genetic detective work has illuminated the important role of Jewish culture in the widespread adoption of citrus fruit by early Mediterranean societies.

The fascinating find came to light in an investigation into a bizarre acidless mutation which makes citrus juice 1000 times less acidic.

John Innes Centre researchers used genetic analysis to trace the acidless mutations in citron, the first citrus species to be cultivated in the Mediterranean.

"Some people thought that this was a recent mutation that originated in Corsica, or somewhere in the Mediterranean, but we have found that this is not new. It's an ancient mutation that is present in Chinese fingered citrons known as Buddha's Hands and those used in the Sukkot Jewish ritual," explains Dr Eugenio Butelli of the John Innes Centre and first author of the paper.

The acidless mutations have captivated botanists and breeders for centuries and appear in many citrus varieties including citron, sweet lime, limetta, lemon and sweet orange.

Acidless citrus fruit have also lost the ability to produce anthocyanin pigments, that give a blush of dark red to leaves, flowers and, sometimes, flesh.

The researchers identified a gene, which they called Noemi, as the key factor behind the regulation of fruit acidity. Analysis also revealed that this gene works in partnership with another, named Ruby, to control anthocyanin production.

The study identified specific mutations affecting the Noemi gene in several acidless citrus species and hybrids. These acidless fruits are often referred to as sweet or insipid because of the reduction in fruit acidity and are highly prized citrons (Etrog in Hebrew) used in the Jewish harvest festival of Sukkot.

One of these mutations matched those found in fingered citron varieties first cultivated in China 3300 years ago. This confirmed that this mutation originated before the arrival of citron into the Mediterranean.

Further analysis revealed that the same ancient Noemi allele characteristic of the acidless trait was present in the Yemen citron, an ancient variety traditionally used in the Sukkot tradition since the time of the destruction of the first temple in 587 B.C.E. Another variety traditionally used in the Sukkot ritual, the Greek citron, also bore the same genetic hallmark.

The analysis suggests that the authentic Jewish Etrog used ritually was an acidless one, an idea supported by a reference to "sweet citron" in the Jewish legal text, the Talmud, dating from 200 C.E.

The study which appears in Current Biology illuminates the path of domestication of citron. It supports the view that the spread of citron in Mediterranean regions was facilitated by its adoption in Jewish culture as an important religious symbol. Some scholars speculate that Jews in exile in Babylonia brought the citron back to Palestine.

Why was this sweet, or insipid citrus, with plain white flowers and leaves drained of colour, the chosen fruit?

"Citron was first cultivated for its medicinal properties in China and its rind was used as a medicinal product, not as a food" explains Professor Cathie Martin of the John Innes Centre and a co-author on the study.

"By the time it reached the Mediterranean in Roman times, citron was a luxury item used for its fragrance to keep linen fresh. The presence of white flowers in the acidless mutation seems important because they are a symbol of purity and we speculate that there was a strong selection for the loss of anthocyanins, which normally add colour to leaves and flowers."

Citron is one of four primary species that make up the citrus genus, a complex group of flowering plants with notable nutritional, medicinal and aromatic value. Despite becoming one of the world's most economically important fruit crops, its history of evolution and domestication has remained obscure until recently.

The characterisation of Noemi provides researchers with an important genetic marker opening a fascinating landscape for genetic analysis of seeds found amid the burials of the ancient world and fossil remains from even further back in time.

The study also gives researchers the information they need to develop fruit of the future - to modulate their level of acidity and to increase their content of health-protecting anthocyanin compounds.

"If you could introduce these mutations stably in lemon, for example, you could make lemonade which does not need so much added sugar in it, making it healthier to drink and better for growing teeth." explains Professor Martin.

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The full study: Noemi controls production of flavonoid pigments and fruit acidity and illustrates the domestication routes of modern citrus varieties, is published in Current Biology.

The full report: https://www.cell.com/current-biology/fulltext/S0960-9822(18)31539-2.

DOI: 10.1016/j.cub.2018.11.040

Pictures/Media and captions: https://drive.google.com/open?id=19h3CEB4uf6L2xN3mrctG7gwGnUyNulp7

Notes for Editors

Contacts

Press Contact: Adrian Galvin - Adrian.Galvin@jic.ac.uk

Tel: 01603 450238/07989 339598

About the John Innes Centre

The John Innes Centre is an independent, international centre of excellence in plant science and microbiology.

Our mission is to generate knowledge of plants and microbes through innovative research, to train scientists for the future, to apply our knowledge of nature's diversity to benefit agriculture, the environment, human health, and wellbeing, and engage with policy makers and the public.

To achieve these goals we establish pioneering long-term research objectives in plant and microbial science, with a focus on genetics. These objectives include promoting the translation of research through partnerships to develop improved crops and to make new products from microbes and plants for human health and other applications. We also create new approaches, technologies and resources that enable research advances and help industry to make new products. The knowledge, resources and trained researchers we generate help global societies address important challenges including providing sufficient and affordable food, making new products for human health and industrial applications, and developing sustainable bio-based manufacturing.

This provides a fertile environment for training the next generation of plant and microbial scientists, many of whom go on to careers in industry and academia, around the world.

The John Innes Centre is strategically funded by the Biotechnology and Biological Sciences Research Council (BBSRC), and is supported by the John Innes Foundation through provision of research accommodation, capital funding and long-term support of the Rotation PhD programme.

For more information about the John Innes Centre visit our website http://www.jic.ac.uk

About BBSRC

The Biotechnology and Biological Sciences Research Council (BBSRC) is part of UK Research and Innovation, a non-departmental public body funded by a grant-in-aid from the UK government.

BBSRC invests in world-class bioscience research and training on behalf of the UK public. Our aim is to further scientific knowledge, to promote economic growth, wealth and job creation and to improve quality of life in the UK and beyond.

Funded by government, BBSRC invested £469 million in world-class bioscience in 2016-17. We support research and training in universities and strategically funded institutes. BBSRC research and the people we fund are helping society to meet major challenges, including food security, green energy and healthier, longer lives. Our investments underpin important UK economic sectors, such as farming, food, industrial biotechnology and pharmaceuticals

For more information about BBSRC, our science and our impact, and for more information about BBSRC strategically funded institutes visit our website.

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