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Pathogens find safe harbor deep in the gastric glands

PLOS

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IMAGE: The gastric glands harbor unique populations of Helicobacter pylori, labeled in this image with two different fluorescent proteins. view more 

Credit: Connie Fung

Scientists have long tried to understand how pathogenic bacteria like Helicobacter pylori, a risk factor for stomach ulcers and cancer, survive in the harsh environment of the stomach. In a new study publishing May 2 in the open-access journal PLOS Biology, researchers led by Connie Fung and Manuel Amieva at Stanford University propose that H. pylori exploit a specialized niche that provides safe harbor deep in the gastric glands to maintain lifelong colonization.

It was already known, from work by the Amieva Lab and others, that H. pylori colonies attached to epithelial cells deep in the gastric glands. This location, the researchers hypothesized, might protect these bacterial colonies from the constant turnover of bacteria at the stomach surface, allowing them to serve as stable bacterial reservoirs.

To determine how H. pylori establish, spread, and persist within the gastric glands, the researchers used high-resolution imaging and mapping techniques to visualize these gland-associated populations in animals colonized with a mixture of H. pylori marked by different fluorescent colors. The infected stomachs were processed using a technique called passive CLARITY, which renders tissues transparent. This allows for intact organs to be imaged in their entirety. The authors discovered that a small number of bacteria act as "founders" that establish within individual gastric glands, replicate, and form colonies. Subsequently, the bacteria spread locally to adjacent glands, forming large clonal population "islands" of the same color that founded the initial gland colony. These population islands persist over time and prevent any incoming bacteria from establishing in the gland space. Consistent with this observation, H. pylori mutant strains that cannot colonize the glands are outcompeted by wild-type bacteria.

These results suggest that a specialized niche in the gastric glands houses a stable H. pylori reservoir that supports chronic infection and may replenish the more transient bacterial populations in the surface mucosa. Similar to their findings with the gastric glands, other groups have also shown that commensal bacteria reside within the intestinal crypts, suggesting that these deeper sites within the gastrointestinal tract may be critical niches for microbial persistence.

"We hope that by understanding the bacterial and host attributes necessary for sustaining these bacterial hideouts, it will lead to therapeutic targets to displace persistent aberrant mucosal colonization," says Manuel Amieva, pediatric infectious disease specialist and senior author of the report. "These principles may extend beyond H. pylori and improve our ability to permanently decolonize patients of bacterial pathogens and antibiotic resistant microbes, and/or replace them with less virulent or beneficial microflora."

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Peer-reviewed / Experimental Study / Cells

In your coverage please use this URL to provide access to the freely available article in PLOS Biology: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000231

Citation: Fung C, Tan S, Nakajima M, Skoog EC, Camarillo-Guerrero LF, Klein JA, et al. (2019) High-resolution mapping reveals that microniches in the gastric glands control Helicobacter pylori colonization of the stomach. PLoS Biol 17(5): e3000231. https://doi.org/10.1371/journal.pbio.3000231

Funding: This research was supported by the American Gastroenterology Association - R. Robert & Sally Funderburg Research Award in Gastric Cancer, the Morgridge Faculty Scholar Award, and the National Institute of Diabetes and Digestive and Kidney Diseases, R01 DK101314 (to M.R.A.); the National Science Foundation Graduate Research Fellowship, DGE - 1147470, and Stanford Graduate Fellowship (to C.F.); the National Institute of Allergy and Infectious Diseases R21 AI137759 (to S.T.); the National Institute of Allergy and Infectious Diseases R01 AI108713 (to J.V.S.); the Wellcome Trust 098051 (to T.D.L.); and the National Science Foundation Dimensions of Biodiversity Program, Award 1737758 (to T.F.). All funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests: The authors have declared that no competing interests exist.

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