News Release 

Brain exosomes from blood samples allow earlier diagnosis of ALS

MicroRNA embedded within small brain particles shed into the bloodstream facilitates early diagnosis of ALS

Brain Chemistry Labs

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IMAGE:  Dr. Sandra Banack uses nanoparticle analyzer to study brain exosomes at the Brain Chemistry Labs, Jackson HLoe. view more 

Credit: Paul Alan Cox

(JACKSON, Wyo.- June 24, 2020) - Exosomes, microscopic packets containing genetic material, are shed by different tissues into the blood. By sequencing microRNA within exosomes originating in the brain, it is now possible to definitively distinguish blood samples of ALS patients from healthy controls, a team of researchers at the Brain Chemistry Labs announced today in Royal Society Open Biology. ALS is a progressive neurodegenerative disease that typically affects people in the prime of their life.

"We think this is a game changer: the methods we have pioneered will lead to the ability to rapidly diagnose ALS from a single blood draw, compared to current scientific measures where patients may have to wait for over a year for a confirmed diagnosis," reports Dr. Sandra Banack, Brain Chemistry Labs Senior Scientist and first author on the new paper. "People with ALS typically live an average of two to three years after diagnosis, so a rapid assessment is crucial."

The new test is based on exosomes, which are microscopic packets containing genetic material that are shed by different tissues in the body. The researchers purified brain exosomes from blood plasma by targeting a unique protein on the exosome surface. Using the brain exosomes, the researchers extracted microRNA, which are short sequences of genetic material that typically regulate gene expression within the cell. Eight different microRNA sequences together form a unique genetic fingerprint that distinguishes blood samples of ALS patients from healthy controls.

According to Dr. Rachael Dunlop, Brain Chemistry Labs Senior Research Fellow, "This new genetic fingerprint may open up opportunities for novel drug discovery. Given the lack of treatments for ALS, physicians and researchers understand the importance of having a new biomarker to help in assessing the effectiveness of new drug candidates, and in enabling patients to receive experimental treatments at an earlier stage of the disease."

Dr. Walter Bradley, Chairman Emeritus of the Department of Neurology at the University of Miami Miller School of Medicine, who was not an author on this study, welcomed this advance. "Early diagnosis is the holy grail for many neurological diseases," he said. "These new results are extremely promising for patients and their physicians."

Translating these new techniques from the laboratory bench to the clinic may still take time, cautions Brain Chemistry Labs Director Dr. Paul Alan Cox. "We are excited by this discovery," Cox explains, "but the analytical techniques at present are costly and time consuming."

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The article, "An miRNA fingerprint using neural-enriched extracellular vesicles from blood plasma: towards a biomarker for amyotrophic lateral sclerosis/motor neuron disease" may be seen online at Royal Society Open Biology (DOI 10.1098/rsob.200116).

About Brain Chemistry Labs

Brain Chemistry Labs is a non-profit research institute based in Jackson Hole, Wyoming which is sponsoring advanced FDA approved clinical trials for ALS and early-stage Alzheimer's disease. With five PhDs currently on staff, the Brain Chemistry Labs anchors a consortium of 50 leading scientists from 28 institutions representing 12 different disciplines. This collaborative model is unique and rapidly advances the search for new cures unencumbered by bureaucracy that often occurs in pharmaceutical firms. For more information about Brain Chemistry Labs, go to https://brainchemistrylabs.org/

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