Diversity in many biological communities is a sign of an ecosystem in balance. When one species dominates, the entire system can go haywire. For example, the uncontrolled overgrowth of certain oceanic algae species causes toxic red tides that kill fish and other sea life, and sicken humans. On a more individual level, the human gut hosts a large community of different bacteria that is crucial for proper digestion and absorption of nutrients. Disruption of or imbalances in this bacterial community can cause a bloom in the growth of a toxic species, causing nausea, diarrhea and other illnesses. Plainly, there's an urgent need to understand how microbial community diversity is developed and maintained, especially as human activities change our external and internal environments.
Like all life, microbes require certain nutrients, such as sunlight, sugars or nitrogen sources, to survive and reproduce. Many microbe species' nutrient requirements overlap, putting them in competition with each other. Much effort has been devoted to understanding how this competition influences microbial diversity when nutrients are steadily supplied. However, in nature, it's quite common for the resources to be available only seasonally so that their supply is severely limited at least some of the time. For example, bacteria in the gut that live on sugars might find these abundant right after the human's had a meal, and scarcer the rest of the time. Because each bacterial species is genetically unique, it will have its own particular strategy for using a given nutrient. Species with the most efficient strategies for using the available nutrients experience the best growth.
"A long-standing question about microbes concerns how so many different microbial species manage to coexist when competing for limited resources," said Ned Wingreen, a professor in Princeton's Department of Molecular Biology and the senior author on a paper in the Sept. 11 issue of the journal eLife.
Researchers can recreate seasonal nutrient supply in a laboratory by placing bacteria in a container with nutrients, letting them grow, then taking a small sample and moving it to a new container of nutrients -- a process called "serial dilution." Over time, the relative abundance of different species in the culture will change according to the nutrients available and the species' nutrient use strategies. By performing repeated rounds of serial dilution, scientists can observe the effects of seasonally supplied nutrients on community diversity.
Of course, it would be impractical to examine all possible combinations of bacteria, nutrients and nutrient utilization strategies using this method. Instead, associate research scholar Amir Erez and graduate student Jaime Lopez, co-authors on the paper and members of Wingreen's lab, and their collaborators investigated this question by mathematically modeling serial dilution.
"In our paper, we develop a general theory of microbial resource competition in a seasonal ecosystem by modeling recurrent nutrient addition and depletion," explained Wingreen.
When nutrients are only seasonally available, the modeling uncovered a surprising relationship between species diversity and the amount of nutrients supplied. First, species diversity is high when all nutrients are supplied in very high amounts because growth of all species is equally unlimited at high nutrient levels. Diversity is also high at very low nutrient amounts because growth of all species is restricted under such conditions and no species can gain an advantage.
"Nutrient-consumption trade-offs in seasonal ecosystems can lead to stable ecosystems that support diversity beyond what is predicted by simpler mathematical models," observed Wingreen.
At intermediate nutrient levels, however, species diversity nose-dives because there will always be one species whose ability to use the most abundant nutrient present outstrips that of others. This species, which the authors call the "early bird," gains an early growth advantage the others can never make up for.
"The early bird species use their earlier access to nutrients to exclude their not-so-early competitors," explains Wingreen. "The early bird is efficient at consuming easily accessible nutrients and uses its early advantage to out-compete competitors for nutrients that are not as easily accessible."
The early bird effect crops up even in more elaborate versions of the model that allow species to feed off others' metabolic byproducts, or for members of a dwindling species to be replenished by in-migration of new individuals. But the identity of the early bird, or whether there will even be one, shifts according to the inputs of the model: what nutrients are present and in what amounts; how often nutrients are supplied; and which species are present and what their strategies are. Whenever it appears, the early bird influences how the ecosystem responds to nutrient changes.
"Ecologists have long sought a universal relationship between biodiversity and the amount of nutrient supplied to a community. The existence of this universal relationship is not supported by our model," says Wingreen.
"This is an important paper," says Alvaro Sanchez, a professor in ecology and evolutionary biology at Yale University and an editor at eLife. "It provides an elegant modeling framework to understand how nutrient supply and competition can structure coexistence and diversity in microbial communities, and it will motivate new experiments."
Funding: This work was supported by NSF grants GM082938, DGE-1656466, PHY-1734030, PHY-1748958, NIH grant R25GM067110, and Gordon and Betty Moore Foundation grant 2919.01.
Citation: Amir Erez, Jaime G. Lopez, Benjamin Weiner, Yigal Meir, and Ned S. Wingreen. Nutrient levels and trade-offs control diversity in a serial dilution ecosystem. eLife.