image: Determination of neural progenitor cell-cell de-adhesion forces using micropipette aspiration view more
Credit: IST Austria
Going from the ventral to the dorsal side, the neural tube, the precursor to the central nervous system, is strikingly organized into domains of cells with different identities and fates. One hypothesis for how this pattern is created is the so-called French Flag model. According to this hypothesis, a signaling molecule sets up a gradient across a field of cells, and a cell "knows" its location within the field, and consequently its identity, by the amount of signaling molecule it receives. However, cells move around as an organism develops, disrupting the strict division into domains. An additional mechanism is needed to maintain the domains. Combining a range of genetic, developmental and biophysical experiments, the groups of Carl-Philipp Heisenberg and Sean Megason, with first author Tony Tsai carrying out experiments in both labs, have been able to solve the puzzle: Adhesion, or the "stickiness" of cells, is the factor that keeps cells of the same domain together, and cells of different domains apart.
Differences in binding induce cell sorting
The Heisenberg lab at IST Austria has developed several biophysical tools with which they can test how strongly cells stick together - for example, by measuring how much force is needed to pull cells apart. Applying this to cells from the zebrafish neural tube, the researchers found that cells belonging to the same domain stick together tightly, while cells from different domains are more easily separated. "This difference in the binding force can induce sorting processes", explains Heisenberg.
The difference in "stickiness" corresponds with a differential expression of adhesion proteins in the domains, the researchers further found. The different cell types express different classes of cadherins: N-cadherin, Cadherin 11, and Protocadherin 19. When the researchers interfere with this expression, making cells express more or less of a certain cadherin, preferential adhesion and correct cell sorting are lost. "Our experiments show that a sorting process based on adhesion, together with a gradient of the signaling molecule Sonic Hedgehog, leads to a precise sorting of cells into domains based on their cell type", concludes Heisenberg. "By combining experiments from biophysics, genetics, and developmental biology, we were able to successfully solve this long-standing question."
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Journal
Science