Microplastic particles exposed to freshwater or saltwater environments for several weeks are about 10 times more likely than pristine particles to be absorbed by mouse cells, due to a crust of microorganisms and biomolecules that forms on the particles' surfaces, according to a new study. The results indicate that this crust acts as a biomolecular "Trojan horse," leading cell membranes to engulf the plastic fragments and usher them into the cell. From there, the particles may infiltrate an organism's circulatory system and tissues, triggering inflammation. Most studies of microplastics' ability to infiltrate living tissues have involved pristine particles, which do not represent the particles found in nature. To investigate how environmental exposure affects how readily microplastic particles can be internalized by cells, Anja Ramsperger and colleagues incubated spherical plastic particles in freshwater from an artificial pond; saltwater from an aquarium; or sterile, ultrapure water for two or four weeks. They then exposed the particles to mouse cells with fluorescently labeled actin, a part of the cytoskeleton involved in particle internalization, for a total of about 3 hours. This labeling enabled easy identification of fully internalized particles, which were entirely surrounded by the glowing actin. Ramsperger et al. observed that microplastic particles exposed to either the freshwater or saltwater environment were internalized by mouse cells 10 times more frequently than pristine particles, and with no significant difference in internalization rates between these non-pristine particle types. These findings suggest that weathered microplastics - such as those in the soupy cesspool of the Great Pacific Garbage Patch - may pose a far greater health risk to organisms than untarnished particles.
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Journal
Science Advances