Optically measured 3D distances (IMAGE)
Caption
Two identical subunits of the bacterial citrate sensor in antiparallel (top) and parallel (bottom) arrangement. Optical 3D position measurements with MINFLUX detect the two states of the dimer formed by the subunits by very precisely measuring the distances between the fluorescent molecules at the protein ends. The ellipses show the measurement uncertainty of the two positions (1, 2 and 3 sigma).
Credit
MPI f. Multidisciplinary Sciences/ Steffen J. Sahl
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Original content