SLC27A5 directly binds to PABPC1 proteins and facilitates its degradation. (IMAGE)
Caption
(A) Proteins interacting with SLC27A5 were detected by IP-MS. APA-related RBPs (PMID: 22502866) and core cleavage/polyadenylation factors and associated RBP (PMID: 25906188) are illustrated in a Venn diagram. (B) Co-immunoprecipitation (Co-IP) of SLC27A5-Flag and PABPC1-HA using an anti-HA antibody (left) or an anti-Flag antibody (right) in HEK-293 cells. (C) Immunoblot analysis of Co-IP by anti-SLC27A5 and anti-PABPC1 in SNU449 cells (left) and HepG2 cells (right). (D) GST pull-down assays of the interaction between SLC27A5 and PABPC1. (E) Immunofluorescence staining to assess the subcellular co-localization of SLC27A5 and PABPC1 in HepG2 cells (scale bar: 20 μm). (F) Immunofluorescence staining was performed using an anti-Flag and anti-HA antibody in PLC/PRF/5 cells transfected with SLC27A5-Flag and PABPC1-HA to detect the subcellular co-localization of SLC27A5 and PABPC1 (scale bar: 20 μm). (G, H) Schematic representation of the SLC27A5 (G) and PABPC1 (H) deletion mutants, and Co-IP assays with the full-length PABPC1 and SLC27A5, respectively. The SLC27A5 deletion mutants covered amino acids 1–77 (ΔC) or 78–690 (ΔN). The PABPC1 deletion mutants covered amino acids 1–370 (ΔC) or 371–636 (ΔN).
Credit
Genes & Diseases
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND