SLC27A5 inhibited HCC stemness by regulating METTL14-APA. (IMAGE)
Caption
(A, B) RT-PCR analysis of stemness-related indicators (A) and sphere-forming assays (B) in MHCC-97H cells infected with AdSLC27A5 (n = 3 independent replicates), ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. (C) Flow-cytometric analysis of the percentage of CD90+ or CD44+ cells in MHCC-97H cells infected with AdSLC27A5. (D) SLC27A5-knockout HepG2 cells transfected with a control vector, or METTL14-US were used to detect stemness-related indicators by qPCR analysis (n = 3 technical replicates). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. (E) Representative images of sphere-forming assays in SLC27A5-KO HepG2 cells transfected with a control vector or METTL14-US. The quantification of spheroids is shown in bar graphs. Scale bar, 5 μm (n = 3 independent replicates). ∗∗p < 0.01; ∗∗∗p < 0.001. (F) Flow-cytometric analysis showing the percentage of CD90+ or CD44+ cells in SLC27A5-KO HepG2 cells transfected with a control vector or METTL14-US. (G) Limiting dilution assay of SLC27A5-KO HepG2 cells stably transfected with a control vector or METTL14-US. Mice were subcutaneously injected with different cell numbers (1 × 105, 1 × 104, 1 × 103). n = 7 per group.
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Genes & Diseases
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