ZW27941 is highly potent in cell cycle progression and inducing apoptosis. (IMAGE)
Caption
(A) Flow cytometric analysis of the cell cycle of MOLM13 cells treated with DMSO, STM2457, UZH2, or ZW27941 (1 μM) for 24 h. The gating strategy is shown in the left upper panel. Cells were stained with BrdU (to determine the S phase) and DAPI (to determine the DNA content). (B) Flow cytometric analysis of the apoptosis of MOLM13 cells in vitro. The gating strategy is shown in the left panel. Cells were stained with annexin V and DAPI. AnnexinV + DAPI− and AnnexinV+ DAPI+cells represent early and late apoptotic cells, respectively. All data are representative of two to three independent experiments. (C) Real-time PCR analysis of MOLM13 cells treated with ZW27941 (1 μM) assessed for relative mRNA expression of several genes. (D) Western blot analysis showed that the c-Myc protein levels were down-regulated in MOLM13 cells after treatment with different concentrations of ZW27941. Data shown were mean ± standard deviation of triplicate experiments; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
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Genes & Diseases
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