Figure 3. Visualization of cartilage and tendons/ligaments using ScxTomato;Sox9EGFP mice (IMAGE)
Caption
In the left panel, a fluorescence stereomicroscopic image shows an optically cleared ScxTomato;Sox9EGFP mouse embryo at E14.5. The central panel presents 3D reconstructed images of the developing paw and distal forearm of a ScxTomato;Sox9EGFP at E16.5, acquired using two-photon excitation fluorescence microscopy and second harmonic generation (SHG) imaging. In this panel, tendons exhibit red fluorescence, cartilage emits green fluorescence, and mature tendons appear light blue due to SHG signals. In the right panel, a cryosection image of the Achilles tendon and calcaneus region from a ScxTomato;Sox9EGFP mouse embryo at E15.5 is shown. The Achilles tendon exhibits red fluorescence, cartilage emits green fluorescence, and yellow cells co-expressing both fluorescent proteins are localized at the attachment site.
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Reproduced with permission from Development, Yu et al., 2025.
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The Company of Biologists, used with permission for press release purposes only.
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