Tetramer interface mutations affect cancer cell proliferation and increase chemotherapy response. (IMAGE)
Caption
(A, B) Relative abundance of NADP+ (A) and NADPH (B) quantified from these reconstituted H520 cell lines (n = 3). (C) Relative reactive oxygen species (ROS) level quantified by flow cytometry (n = 3). (D, E) The reconstituted H520 cells were labeled with 10 μM EdU for 30 min, and EdU-positive cells were examined by immunostaining test (D). EdU-positive cells are presented in (E) (n = 5). Scale bars: 50 μm. (F) The reconstituted H520 cells were exposed to indicated concentrations of cisplatin for 96 h, and cell viability assay was performed (n = 4). The IC50 of cisplatin was calculated. (G–I) Mice were subcutaneously injected with 1 × 106 of the indicated H520 cells on both sides of the armpit (n = 5). When tumors were visible, mice were treated with cisplatin (2 mg/kg) every 3 days for 2 weeks. (G) The tumors were harvested after an additional 7 days of growth. (H, I) Relative tumor volume (H) and tumor weight ratio (cisplatin/ctrl) (I) are presented. Data in (A–F, I) are presented as mean ± standard deviation; one-way ANOVA; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. Data in (H) are presented as mean ± standard deviation; two-way ANOVA; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.
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Genes & Diseases
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