Example of multimodal molecular characterization of human kidney tissue. (IMAGE)
Caption
Whole slide microscopy images from donor VAN0028 (56-year-old white female) were collected using AF (A.a and A.b) before high-spatial-resolution (10-μm pixel size) MALDI IMS measurement, and PAS-stained microscopy (A.c) data were acquired post-IMS. AF microscopy data were automatically segmented into renal FTUs (A.b), including the GLs (green), PTs (magenta), TAL (light green), DTs (brown), and CDs (red). MALDI IMS measurement regions (white boxes) were selected to include a mixture of tissue features. The microscopy data for the MALDI IMS measurement region are highlighted in (A.d) to (A.f). Selected individual molecular distribution images from the negative ion mode MALDI IMS measurement and an overlay image are provided in (B.a) to (B.d) and (B.e), respectively. The selected ions demonstrate unique localizations within the kidney without the need for prior labeling, and these are just four of the hundreds of lipids that make up this molecular atlas. The mean mass spectrum associated with each FTU, obtained by averaging FTU-specific IMS-pixels across all donors, displays subtle differences in the lipids detected and their intensities (B.f). It is noted that full size versions of the spectra can be found in the Supplementary Materials. Variations in intensity profiles of FTUs are more evident using difference spectra, as shown in the comparison between the normalized (between 0 and 1 to allow for direct comparison) average spectrum of PTs subtracted from that of the GLs (B.g).
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Farrow et. al
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