Metformin rescued RO and mouse retinal function after NaIO3 induction. (IMAGE)
Caption
(A) TUNEL assay of control, control+metformin, NaIO3 injury, NaIO3 injury+metformin and NaIO3 injury+metformin organoids 7 day pretreatment groups. No TUNEL-positive cells (green) were observed in the control, control+metformin or NaIO3 injury+metformin 7 day pretreatment groups. Many TUNEL-positive cells were observed in the NaIO3 injury group, whereas few TUNEL-positive cells were observed in the NaIO3 injury+metformin group. (B) Quantification of TUNEL in the control, control+metformin, NaIO3 injury, NaIO3 injury+metformin and NaIO3 injury+metformin RO 7 day pretreatment groups (n=6/group). (C) H&E staining of retinas from the control, control+metformin, NaIO3 injury, NaIO3 injury+metformin and NaIO3 injury+metformin 7 day pretreatment groups. (D) Quantification of ONL thickness in the retinas of the control, control+metformin, NaIO3 injury, NaIO3 injury+metformin and NaIO3 injury+metformin 7 day pretreatment groups (n=6/group). (E) ERG analysis of retinal function in the control, control+metformin, NaIO3 injury, NaIO3 injury+metformin and NaIO3 injury+metformin 7 day pretreatment groups. (F, G) Bar plot of the amplitudes of a and b waves in the control, control+metformin, NaIO3 injury, NaIO3 injury+metformin and NaIO3 injury+metformin 7 day pretreatment groups (n=6/group). (H) Flow cytometry analysis of retinal apoptosis in the NaIO3 injury, NaIO3 injury+metformin and NaIO3 injury+metformin 7 day pretreatment groups. (n=6/group). (Data are shown as the mean ± SD. Statistical analyses are determined by One-way ANOVA, followed by Tukey’s test. ***P < 0.001).
Credit
Xiaolu Hao, Lu Du, Guang Liu, Zhaohui Li, Shaojun Wang
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